| Vesicular stomatitis virus-mediated cell fusion subsequent to virus adsorption at different pH values. | |
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MedLine Citation:
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PMID: 1668239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vesicular stomatitis virus (VSV)-mediated cell fusion from without can be induced by transient exposure to low pH, subsequent to adsorption of VSV at neutral pH. To study the mechanism of VSV-induced cell fusion, we examined the effect of pH condition at virus adsorption on acid-inducible VSV-mediated cell fusion. Although the binding of VSV to BHK-21 cells was most efficient under acidic condition (pH 5.7-6.3), extensive cell fusion was not observed under this condition. A temporary exposure to low pH after binding at neutral pH also decreased fusion activity. However, return to neutral pH for 2 min just after the acid binding restored the fusion activity. These results indicate the requirement of neutral pH condition for VSV-mediated cell fusion prior to the acid stimulation which induces conformational change of the virus glycoprotein into a fusogenic form. |
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Authors:
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S Nagata; T Kurata; Y Ueno; J Chiba |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Japanese journal of medical science & biology Volume: 44 ISSN: 0021-5112 ISO Abbreviation: Jpn. J. Med. Sci. Biol. Publication Date: 1991 Aug |
Date Detail:
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Created Date: 1992-08-20 Completed Date: 1992-08-20 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0243706 Medline TA: Jpn J Med Sci Biol Country: JAPAN |
Other Details:
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Languages: eng Pagination: 171-80 Citation Subset: IM |
Affiliation:
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Department of Pathology, National Institute of Health, Tokyo. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals Cell Fusion / physiology* Cell Line Hydrogen-Ion Concentration Membrane Fusion / physiology Vesicular stomatitis Indiana virus / physiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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