Document Detail


Verticillium dahliae toxin induced alterations of cytoskeletons and nucleoli in Arabidopsis thaliana suspension cells.
MedLine Citation:
PMID:  17019529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In plant cells, cytoskeletons play important roles in response to biotic and abiotic stresses. However, little is known about the dynamics of cytoskeletons when cells are attacked by unphysical stress factors such as elicitors and toxins. We report here that the toxin of Verticillium dahliae (VD toxin) induced changes of microfilaments (MFs) and microtubules (MTs) in Arabidopsis thaliana suspension-cultured cells. When cells were treated with a low concentration of VD toxin, MFs were disrupted ordinally from the cortex to the perinuclear region, and then recovered spontaneously; but the MTs persisted. The MFs in the perinuclear region showed more resistance to VD toxin than the cortical ones. In contrast, when cells were treated with a high concentration of VD toxin, MFs and MTs were disrupted sooner and more severely and did not recover spontaneously. Treatments with high concentrations of VD toxin also induced changes of nucleoli. At the early stages of treatment, a nucleus had a single ring-shaped nucleolus. At the later stages, multiple smaller and more brightly fluorescing nucleoli emerged in a single nucleus. Disrupted MFs could be recovered by removing the VD toxin before the ring-shaped nucleoli appeared. All these results showed that MFs and MTs play important roles in the early defense responses against VD toxin in Arabidopsis suspension cells. The cytoskeletons may be used as sensors and effectors monitoring the defense reactions. The changes of nucleoli induced by VD toxin should be important characteristics of cell death.
Authors:
Hai-Yong Yuan; Lin-Lin Yao; Zhi-Qi Jia; Yun Li; Ying-Zhang Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-06
Journal Detail:
Title:  Protoplasma     Volume:  229     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-14     Completed Date:  2007-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  75-82     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology,  drug effects*,  metabolism
Cell Nucleolus / metabolism*
Cells, Cultured
Cytoskeleton / metabolism*
Dose-Response Relationship, Drug
Microfilaments / metabolism
Microscopy, Confocal
Microtubules / metabolism
Mycotoxins / pharmacology*
Verticillium / chemistry*
Chemical
Reg. No./Substance:
0/Mycotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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