| Vertical profiles of methanogenesis and methanogens in two contrasting acidic peatlands in central New York State, USA. | |
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MedLine Citation:
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PMID: 16872405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Northern acidic peatlands are important sources of atmospheric methane, yet the methanogens in them are poorly characterized. We examined methanogenic activities and methanogen populations at different depths in two peatlands, McLean bog (MB) and Chicago bog (CB). Both have acidic (pH 3.5-4.5) peat soils, but the pH of the deeper layers of CB is near-neutral, reflecting its previous existence as a neutral-pH fen. Acetotrophic and hydrogenotrophic methanogenesis could be stimulated in upper samples from both bogs, and phylotypes of methanogens using H2/CO2 (Methanomicrobiales) or acetate (Methanosarcinales) were identified in 16S rRNA gene clone libraries and by terminal restriction fragment length polymorphism (T-RFLP) analyses using a novel primer/restriction enzyme set that we developed. Particularly dominant in the upper layers was a clade in the Methanomicrobiales, called E2 here and the R10 or fen group elsewhere, estimated by quantitative polymerase chain reaction to be present at approximately 10(8) cells per gram of dry peat. Methanogenic activity was considerably lower in deeper samples from both bogs. The methanogen populations detected by T-RFLP in deeper portions of MB were mainly E2 and the uncultured euryarchaeal rice cluster (RC)-II group, whereas populations in the less acidic CB deep layers were considerably different, and included a Methanomicrobiales clade we call E1-E1', as well as RC-I, RC-II, marine benthic group D, and a new cluster that we call the subaqueous cluster. E2 was barely detectable in the deeper samples from CB, further evidence for the associations of most organisms in this group with acidic habitats. |
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Authors:
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Hinsby Cadillo-Quiroz; Suzanna Bräuer; Erika Yashiro; Christine Sun; Joseph Yavitt; Stephen Zinder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Environmental microbiology Volume: 8 ISSN: 1462-2912 ISO Abbreviation: Environ. Microbiol. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-28 Completed Date: 2006-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883692 Medline TA: Environ Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1428-40 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Cornell University, Ithaca, NY, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Colony Count, Microbial
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methods Ecosystem Methane / metabolism* Methanomicrobiales / genetics, isolation & purification, metabolism* Methanosarcinales / genetics, isolation & purification, metabolism* New York Phylogeny Polymorphism, Restriction Fragment Length Proton-Motive Force / physiology RNA, Ribosomal, 16S / genetics Soil* / analysis Soil Microbiology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Ribosomal, 16S; 0/Soil; 74-82-8/Methane |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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