| Vertical nanopillars for highly localized fluorescence imaging. | |
| | |
MedLine Citation:
|
PMID: 21368157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Observing individual molecules in a complex environment by fluorescence microscopy is becoming increasingly important in biological and medical research, for which critical reduction of observation volume is required. Here, we demonstrate the use of vertically aligned silicon dioxide nanopillars to achieve below-the-diffraction-limit observation volume in vitro and inside live cells. With a diameter much smaller than the wavelength of visible light, a transparent silicon dioxide nanopillar embedded in a nontransparent substrate restricts the propagation of light and affords evanescence wave excitation along its vertical surface. This effect creates highly confined illumination volume that selectively excites fluorescence molecules in the vicinity of the nanopillar. We show that this nanopillar illumination can be used for in vitro single-molecule detection at high fluorophore concentrations. In addition, we demonstrate that vertical nanopillars interface tightly with live cells and function as highly localized light sources inside the cell. Furthermore, specific chemical modification of the nanopillar surface makes it possible to locally recruit proteins of interest and simultaneously observe their behavior within the complex, crowded environment of the cell. |
| | |
Authors:
|
Chong Xie; Lindsey Hanson; Yi Cui; Bianxiao Cui |
Related Documents
:
|
10445057 - Distortion-product otoacoustic emissions (dpes) in neonates: frequency ratio (f2/f1) an... 9799987 - Comparison of distortion-product and transient evoked otoacoustic emissions with abr th... 1578277 - On the frequency limit and phase of outer hair cell motility: effects of the membrane f... 16938977 - Distortion-product otoacoustic emission suppression growth in normal and noise-exposed ... 2214727 - Mechanical characteristics of micro-switches adapted for the physically disabled. 20836447 - Overrun by the neighbors: landscape context affects strength and sign of local adaptation. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-22 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-03-09 Completed Date: 2011-05-26 Revised Date: 2011-09-13 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 3894-9 Citation Subset: IM |
Affiliation:
|
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Microscopy, Fluorescence
/
methods* Nanostructures* Silicon Dioxide / chemistry |
| Grant Support | |
ID/Acronym/Agency:
|
NS057906/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
7631-86-9/Silicon Dioxide |
| Comments/Corrections | |
Comment In:
|
Nat Methods. 2011 Apr;8(4):284-5
[PMID:
21574270
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Redesigning allosteric activation in an enzyme.
Next Document: Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane protei...