| Versatile derivatisation of solid support media for covalent bonding on DNA-microchips. | |
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MedLine Citation:
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PMID: 10198429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A chemistry was developed that permits on DNA-arrays both the covalent immobilisation of pre-fabricated nucleic acids-such as oligonucleotides, PCR-products or peptide nucleic acid oligomers-and the in situ synthesis of such compounds on either glass or polypropylene surfaces. Bonding was found to be stable even after some 30 cycles of stripping. Due to a dendrimeric structure of the linker molecule, the loading can be modified in a controlled manner and increased beyond the capacity of glass without negative effects on hybridisation efficiency. Also, the chemistry warrants the modulation of other surface properties such as charge or hydrophobicity. Preferentially, attachment of nucleic acids takes place only via the terminal amino-group of amino-modified oligonucleotides or the terminal hydroxyl-group of unmodified molecules so that the entire molecule is accessible to probe hybridisation. This derivatisation represents a support chemistry versatile enough to serve nearly all current forms of DNA-arrays or microchips. |
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Authors:
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M Beier; J D Hoheisel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleic acids research Volume: 27 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-06-14 Completed Date: 1999-06-14 Revised Date: 2008-11-20 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1970-7 Citation Subset: IM |
Affiliation:
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Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany. m.beier@dkfz-heidelberg.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Biopolymers / chemistry DNA / chemistry* Miniaturization* Oligonucleotide Array Sequence Analysis Peptide Nucleic Acids / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Biopolymers; 0/Peptide Nucleic Acids; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 1 Month: 5 Year: 1999 Volume: 27 Issue: 9 First Page: 1970 Last Page: 1977 ID: 148409 PubMed Id: 10198429 Publisher Item Identifier: gkc338 |
| Versatile derivatisation of solid support media for covalent bonding on DNA-microchips. | |
| M Beier | |
| J D Hoheisel | |
| Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany. m.beier@dkfz-heidelberg.de |
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