Document Detail


Versatile copurification procedure for rapid isolation of homogeneous RAR-RXR heterodimers.
MedLine Citation:
PMID:  10419826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RAR-RXR heterodimeric complexes (RARalphaDeltaAB-RXRalphaDeltaAB) bound to cognate DR5 DNA response elements were purified to apparent structural and functional homogeneity using a novel versatile immobilized metal affinity chromatography (IMAC) copurification procedure. Dynamic light scattering studies indicated that the complexes were more than 85% monodisperse. By electron microscopy the negatively stained RAR-RXR-DNA complexes appeared homogeneous and corresponded to a dimeric arrangement of the molecules. Using heterodimers purified according to this procedure we demonstrate ligand binding of RXR in the context of the RAR-RXR heterodimer-DNA complex. The present copurification procedure is rapid and has yielded high quality heterodimer-DNA complexes suitable for both structural and biochemical studies.
Authors:
J Iyer; D Bonnier; F Granger; C Iltis; V Andry; P Schultz; H Gronemeyer
Related Documents :
16705166 - Mal and ternary complex factor use different mechanisms to contact a common surface on ...
4084556 - Sequence dependence of the curvature of dna: a test of the phasing hypothesis.
3713816 - Sequence-directed curvature of dna.
16720606 - Effect of eyespot caused by oculimacula yallundae and o. acuformis, assessed visually a...
22414726 - Activation of dna damage response pathways as a consequence of anthracycline-dna adduct...
4919986 - Studies on the pathway of incorporation of 2-aminopurine into the deoxyribonucleic acid...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein expression and purification     Volume:  16     ISSN:  1046-5928     ISO Abbreviation:  Protein Expr. Purif.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-18     Completed Date:  1999-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9101496     Medline TA:  Protein Expr Purif     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  308-14     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC)/CNRS/INSERM/ULP, C.U. de Strasbourg, Illkirch Cedex, 67404, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, Affinity
Dimerization
Electrophoresis, Polyacrylamide Gel
Ligands
Microscopy, Electron
Receptors, Retinoic Acid / chemistry,  isolation & purification*,  metabolism
Recombinant Proteins / chemistry,  isolation & purification,  metabolism
Retinoid X Receptors
Transcription Factors / chemistry,  isolation & purification*,  metabolism
Chemical
Reg. No./Substance:
0/Ligands; 0/Receptors, Retinoic Acid; 0/Recombinant Proteins; 0/Retinoid X Receptors; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression and purification of recombinant human zona pellucida proteins.
Next Document:  Use of the green fluorescent protein variant (YFP) to monitor MetArg human proinsulin production in ...