Document Detail

Verotoxin-binding in human renal sections.
MedLine Citation:
PMID:  8107950     Owner:  NLM     Status:  MEDLINE    
Gastrointestinal infection with verotoxin-producing Escherichia coli (VTEC) has been strongly implicated in the etiology of the hemolytic uremic syndrome (HUS), the leading cause of pediatric acute renal failure. The binding of fluorescein-conjugated VT1 overlaid on to frozen human renal sections has been examined. Sections from biopsies of infants aged < 2 years were compared with those from adult autopsies. VT primarily stained distal convoluted tubules, particularly those adjacent to glomeruli, and collecting ducts. VT-binding was detected within the infant glomerulus but not the adult. Binding of the toxin was removed when the section was pretreated with alpha-galactosidase, confirming the receptor-binding specificity for globotriaosyl ceramide (gal alpha 1-4gal beta 1-4 glucosylceramide), the glycolipid receptor for VT. These studies may suggest that differential localization of this glycolipid in the pediatric renal glomerulus is a risk factor for the development of HUS following infection with VTEC.
C A Lingwood
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nephron     Volume:  66     ISSN:  0028-2766     ISO Abbreviation:  Nephron     Publication Date:  1994  
Date Detail:
Created Date:  1994-03-18     Completed Date:  1994-03-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0331777     Medline TA:  Nephron     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  21-8     Citation Subset:  IM    
Department of Microbiology, Hospital for Sick Children, Toronto, Ont., Canada.
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MeSH Terms
Age Factors
Bacterial Toxins / metabolism*
Carbohydrate Sequence
Child, Preschool
Escherichia coli Infections / complications
Gastrointestinal Diseases / complications
Glycolipids / chemistry,  metabolism
Hemolytic-Uremic Syndrome / etiology,  metabolism
Hepatorenal Syndrome / etiology,  metabolism
Kidney / metabolism*
Molecular Sequence Data
Nephrosis, Lipoid / etiology,  metabolism
Receptors, Cell Surface / chemistry,  drug effects,  metabolism
Shiga Toxin 1
Trihexosylceramides / chemistry,  metabolism
alpha-Galactosidase / pharmacology
Reg. No./Substance:
0/Bacterial Toxins; 0/Glycolipids; 0/Receptors, Cell Surface; 0/Shiga Toxin 1; 0/Shiga-like toxin receptor; 0/Trihexosylceramides; 71965-57-6/globotriaosylceramide; EC

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