Document Detail

Verification of an Edwardsiella ictaluri-specific diagnostic PCR.
MedLine Citation:
PMID:  20002568     Owner:  NLM     Status:  MEDLINE    
AIMS: To verify the specificity of a PCR assay for the identification and diagnosis of Edwardsiella ictaluri. METHODS AND RESULTS: An Edwardsiella ictaluri-specific PCR assay was developed utilizing two features of the ribosomal DNA gene clusters. The first feature is the presence of two ribosomal gene clusters located in tandem to one another (the inter-ribosomal spacer, IRS). This characteristic is present in the Edwardsiella genus but absent in the other sequenced members of the Enterobacteriaceae. The second feature is the presence of an intervening sequence (IVS) in the 23S rRNA gene of Edw. ictaluri. To verify the specificity of this assay, we tested genomic DNA from a variety of bacterial species. The IVS/IRS PCR assay results in an c. 2000-bp product from all Edw. ictaluri isolates tested, but not from any other species including Edwardsiella tarda. CONCLUSIONS: The IVS/IRS PCR assay is highly specific for Edw. ictaluri and useful as a tool for identifying this pathogen. SIGNIFICANCE AND IMPACT OF THE STUDY: This research verifies the specificity of PCR-based assay for Edw. Ictaluri, and we describe this assay as a highly versatile diagnostic tool for its identification.
M L Williams; M L Lawrence
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-11-06
Journal Detail:
Title:  Letters in applied microbiology     Volume:  50     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  153-7     Citation Subset:  IM    
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
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MeSH Terms
Catfishes / microbiology
DNA, Bacterial / genetics*
DNA, Ribosomal Spacer / genetics*
Edwardsiella ictaluri / genetics*,  isolation & purification
Edwardsiella tarda / genetics
Fish Diseases / diagnosis,  genetics,  microbiology
Multigene Family / genetics*
Polymerase Chain Reaction / methods*
RNA, Bacterial / genetics
RNA, Ribosomal, 23S / genetics
Sensitivity and Specificity
Species Specificity
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal Spacer; 0/RNA, Bacterial; 0/RNA, Ribosomal, 23S

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