| Verapamil enhances the antitumoral efficacy of oncolytic adenoviruses. | |
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MedLine Citation:
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PMID: 20179683 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The therapeutic potential of oncolytic adenoviruses is limited by the rate of adenovirus release. Based on the observation that several viruses induce cell death and progeny release by disrupting intracellular calcium homeostasis, we hypothesized that the alteration in intracellular calcium concentration induced by verapamil could improve the rate of virus release and spread, eventually enhancing the antitumoral activity of oncolytic adenoviruses. Our results indicate that verapamil substantially enhanced the release of adenovirus from a variety of cell types resulting in an improved cell-to-cell spread and cytotoxicity. Furthermore, the combination of the systemic administration of an oncolytic adenovirus (ICOVIR-5) with verapamil in vivo greatly improved its antitumoral activity in two different tumor xenograft models without affecting the selectivity of this virus. Overall, our findings indicate that verapamil provides a new, safe, and versatile way to improve the antitumoral potency of oncolytic adenoviruses in the clinical setting. |
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Authors:
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Alena Gros; Cristina Puig; Sonia Guedan; Juan José Rojas; Ramon Alemany; Manel Cascallo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-23 |
Journal Detail:
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Title: Molecular therapy : the journal of the American Society of Gene Therapy Volume: 18 ISSN: 1525-0024 ISO Abbreviation: Mol. Ther. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-07-30 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 100890581 Medline TA: Mol Ther Country: United States |
Other Details:
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Languages: eng Pagination: 903-11 Citation Subset: IM |
Affiliation:
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Translational Research Laboratory, IDIBELL-Institut Català d'Oncologia, L'Hospitalet de Llobregat, Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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drug effects,
genetics,
physiology* Animals Blotting, Western Cell Line Cell Line, Tumor Genetic Vectors Humans Male Mice Mice, Inbred BALB C Neoplasms / therapy Oncolytic Virotherapy / methods* Oncolytic Viruses / drug effects, genetics, physiology* Verapamil / therapeutic use* Virus Release / drug effects Xenograft Model Antitumor Assays |
| Chemical | |
Reg. No./Substance:
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52-53-9/Verapamil |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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