Document Detail

Ventromedial hypothalamic lesion-induced vagal hyperactivity stimulates rat pancreatic cell proliferation.
MedLine Citation:
PMID:  8608899     Owner:  NLM     Status:  MEDLINE    
BACKGROUND & AIMS: Ventromedial hypothalamic (VMH) lesions cause an increase in DNA content in the rat pancreas. This study examined the role of cell proliferation in the mitotic response of the rat pancreas after VMH lesion formation. METHODS: Alterations in rat pancreatic DNA content, DNA synthesis, and labeling indices using antiproliferation cell nuclear antigen molecular antibody were measured 0,1,3, and 7 days after VMH lesion formation. Additionally, the effects of vagotomy, atropine, or anti-insulin antibody on VMH lesion-induced alterations in DNA synthesis were examined. Pancreatic samples were also treated with double immunostaining: first for PCNA and then for insulin, glucagon, and somatostatin. RESULTS: Pancreatic weight, DNA content, and DNA synthesis increased in animals receiving VMH lesions. Proliferation was primarily observed in islet B and acinar cells beginning 1 day after VMH lesion formation, reaching a maximum rate after 3 days. VMH lesion-induced stimulation of DNA synthesis was completely inhibited by vagotomy or atropine administration but not by anti-insulin antibody. CONCLUSIONS: Vagal hyperactivity produced by VMH lesions stimulated cell proliferation of rat pancreatic islet B and acinar cells primarily through a cholinergic receptor mechanism.
T Kiba; K Tanaka; K Numata; M Hoshino; K Misugi; S Inoue
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gastroenterology     Volume:  110     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-05-30     Completed Date:  1996-05-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  885-93     Citation Subset:  AIM; IM    
Third Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
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MeSH Terms
Analysis of Variance
Atropine / pharmacology
Cell Division / drug effects
DNA / biosynthesis,  metabolism
Insulin / blood
Insulin Antibodies / pharmacology
Pancreas / drug effects,  metabolism,  pathology*
Parasympatholytics / pharmacology
Rats, Sprague-Dawley
Vagus Nerve / physiopathology*
Ventromedial Hypothalamic Nucleus / pathology,  surgery*
Reg. No./Substance:
0/Insulin Antibodies; 0/Parasympatholytics; 11061-68-0/Insulin; 51-55-8/Atropine; 9007-49-2/DNA

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