Document Detail

Ventricular tachyarrhythmias in rats with acute myocardial infarction involves activation of small-conductance Ca2+-activated K+ channels.
MedLine Citation:
PMID:  23086994     Owner:  NLM     Status:  MEDLINE    
In vitro experiments have shown that the upregulation of small-conductance Ca(2+)-activated K(+) (SK) channels in ventricular epicardial myocytes is responsible for spontaneous ventricular fibrillation (VF) in failing ventricles. However, the role of SK channels in regulating VF has not yet been described in in vivo acute myocardial infarction (AMI) animals. The present study determined the role of SK channels in regulating spontaneous sustained ventricular tachycardia (SVT) and VF, the inducibility of ventricular tachyarrhythmias, and the effect of inhibition of SK channels on spontaneous SVT/VF and electrical ventricular instability in AMI rats. AMI was induced by ligation of the left anterior descending coronary artery in anesthetized rats. Spontaneous SVT/VF was analyzed, and programmed electrical stimulation was performed to evaluate the inducibility of ventricular tachyarrhythmias, ventricular effective refractory period (VERP), and VF threshold (VFT). In AMI, the duration and episodes of spontaneous SVT/VF were increased, and the inducibility of ventricular tachyarrhythmias was elevated. Pretreatment in the AMI group with the SK channel blocker apamin or UCL-1684 significantly reduced SVT/VF and inducibility of ventricular tachyarrhythmias (P < 0.05). Various doses of apamin (7.5, 22.5, 37.5, and 75.0 μg/kg iv) inhibited SVT/VF and the inducibility of ventricular tachyarrhythmias in a dose-dependent manner. Notably, no effects were observed in sham-operated controls. Additionally, VERP was shortened in AMI animals. Pretreatment in AMI animals with the SK channel blocker significantly prolonged VERP (P < 0.05). No effects were observed in sham-operated controls. Furthermore, VFT was reduced in AMI animals, and block of SK channels increased VFT in AMI animals, but, again, this was without effect in sham-operated controls. Finally, the monophasic action potential duration at 90% repolarization (MAPD(90)) was examined in the myocardial infarcted (MI) and nonmyocardial infarcted areas (NMI) of the left ventricular epicardium. Electrophysiology recordings showed that MAPD(90) in the MI area was shortened in AMI animals, and pretreatment with SK channel blocker apamin or UCL-1684 significantly prolonged MAPD(90) (P < 0.05) in the MI area but was without effect in the NMI area or in sham-operated controls. We conclude that the activation of SK channels may underlie the mechanisms of spontaneous SVT/VF and susceptibility to ventricular tachyarrhythmias in AMI. Inhibition of SK channels normalized the shortening of MAPD(90) in the MI area, which may contribute to the inhibitory effect on spontaneous SVT/VF and inducibility of ventricular tachyarrhythmias in AMI.
Le Gui; Zhiwei Bao; Yinyu Jia; Xiaotong Qin; Zixi Jack Cheng; Jianhua Zhu; Qing-Hui Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-19
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  304     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-02-20     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H118-30     Citation Subset:  IM    
Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
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MeSH Terms
Action Potentials
Alkanes / pharmacology
Anti-Arrhythmia Agents / pharmacology
Apamin / pharmacology
Cardiac Pacing, Artificial
Disease Models, Animal
Dose-Response Relationship, Drug
Heart Rate
Myocardial Infarction / complications*,  drug therapy,  genetics,  metabolism,  physiopathology
Potassium Channel Blockers / pharmacology
Quinolinium Compounds / pharmacology
Rats, Sprague-Dawley
Refractory Period, Electrophysiological
Small-Conductance Calcium-Activated Potassium Channels / drug effects,  genetics,  metabolism*
Tachycardia, Ventricular / etiology*,  genetics,  metabolism,  physiopathology,  prevention & control
Time Factors
Ventricular Fibrillation / etiology*,  genetics,  metabolism,  physiopathology,  prevention & control
Reg. No./Substance:
0/6,10-diaza-3(1,3),8(1,4)dibenzena-1,5(1,4)diquinolinacyclodecaphane; 0/Alkanes; 0/Anti-Arrhythmia Agents; 0/Kcnn1 protein, rat; 0/Kcnn2 protein, rat; 0/Kcnn3 protein, rat; 0/Potassium Channel Blockers; 0/Quinolinium Compounds; 0/Small-Conductance Calcium-Activated Potassium Channels; 24345-16-2/Apamin

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