Document Detail

Ventilatory behavior and carotid body morphology of Brown Norway and Sprague Dawley rats.
MedLine Citation:
PMID:  21729771     Owner:  NLM     Status:  MEDLINE    
Differences in acute ventilatory behavior are associated with carotid body (CB) structural and immunohistologic profiles in some, but not all, reports. Brown Norway (BN) rats exhibit lower acute ventilatory responses to hypoxia and hypercapnia compared to Sprague Dawley (SD) rats. We hypothesized that BN rats possess CB with fewer glomus cells. Ventilation was recorded in 6-month-old BN and SD rats exposed to hypoxia-reoxygenation and hypercapnia. Extracted CBs were examined using H&E staining, and immunohistochemistry with antibodies specific for tyrosine hydroxylase (TH), neural nitric oxide synthase (nNOS), and pyruvate dehydrogenase (PD). Sections were analyzed for cell and immunostaining density. SD displayed greater hypoxic and hypercapnic responses, and post-hypoxic short term potentiation, whereas BN exhibited post-hypoxic frequency decline. Contrary to our hypothesis, BN demonstrated a denser arrangement of glomus cells with a larger TH stained area (31.7% BN, 22.6% SD; p<0.0001), and nNOS stained area (37.3% BN, 32.1%; SD; p=0.01). Hence, respiratory phenotype does not correlate intuitively with these anatomic features.
Lucas M Donovan; Sam Chai; Carl B Gillombardo; Steven N Emancipator; Kingman P Strohl
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-06-25
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  178     ISSN:  1878-1519     ISO Abbreviation:  Respir Physiol Neurobiol     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-29     Completed Date:  2012-06-11     Revised Date:  2013-02-11    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  250-5     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carotid Body / anatomy & histology*,  physiology*
Pulmonary Ventilation / physiology*
Rats, Inbred BN
Rats, Sprague-Dawley
Species Specificity
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Systemic inflammation impairs respiratory chemoreflexes and plasticity.
Next Document:  Chest wall kinematics and breathlessness during unsupported arm exercise in COPD patients.