Document Detail


Venous drainage in retrograde island flap: an experimental study using fluorescence tracing technique.
MedLine Citation:
PMID:  19670241     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pathway of venous drainage in retrograde island flaps was investigated by fluorescence tracing technique using the saphenous fasciocutaneous flap in New Zealand White rabbits. Forty animals were allocated into four groups according to the different times at 30 minutes (I), 24 hours (II), 72 hours (III), and 7 days (IV) after the operation. According to the different routes to give tracer, each group was further allocated into two subgroups of the artery injection and vein injection. For each animal, one hindlimb was assigned as the experimental side, the contralateral side as control without giving tracer. The erythrocytes were separated, labeled with fluorescein isothiocyanate (FITC), detected, and injected into the artery or vein. Subsequently, the flaps were harvested 5 seconds after injection and immediately frozen, sectioned, and observed under microscope. In group I and II, the fluorescence was observed mainly around the vessel adventitia of the vein and artery and tunica intima of the artery. In group III, there was weak fluorescence observed in the lumen of vein. In group IV, fluorescence was distributed principally in the lumen of the vein. In addition, fluorescence was not observed in the saphenous nerve in group I and there was mild fluorescence in the saphenous nerve in groups II, III, and IV. These findings suggest that the venous return is through "bypass route" in earlier period. In later period, the venous retrograde return is through "bypass route" and "incompetent valves route;" however, "incompetent valves route" becomes the main route.
Authors:
Zhi-Zhen Jing; Shi-Min Chang; Mu-Rong You; Guang-Rong Yu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microsurgery     Volume:  30     ISSN:  1098-2752     ISO Abbreviation:  Microsurgery     Publication Date:  2010  
Date Detail:
Created Date:  2010-01-11     Completed Date:  2010-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309230     Medline TA:  Microsurgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  50-4     Citation Subset:  IM    
Affiliation:
The Department of Orthopedic Surgery, Tongji Hospital, Tongji University, Shanghai, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fluorescein-5-isothiocyanate / diagnostic use*
Fluorescent Dyes / diagnostic use*
Hindlimb / blood supply*
Microcirculation / physiology
Microscopy, Fluorescence*
Rabbits
Regional Blood Flow / physiology
Saphenous Vein*
Surgical Flaps / blood supply*
Time Factors
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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