| Venous drainage in retrograde island flap: an experimental study using fluorescence tracing technique. | |
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MedLine Citation:
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PMID: 19670241 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pathway of venous drainage in retrograde island flaps was investigated by fluorescence tracing technique using the saphenous fasciocutaneous flap in New Zealand White rabbits. Forty animals were allocated into four groups according to the different times at 30 minutes (I), 24 hours (II), 72 hours (III), and 7 days (IV) after the operation. According to the different routes to give tracer, each group was further allocated into two subgroups of the artery injection and vein injection. For each animal, one hindlimb was assigned as the experimental side, the contralateral side as control without giving tracer. The erythrocytes were separated, labeled with fluorescein isothiocyanate (FITC), detected, and injected into the artery or vein. Subsequently, the flaps were harvested 5 seconds after injection and immediately frozen, sectioned, and observed under microscope. In group I and II, the fluorescence was observed mainly around the vessel adventitia of the vein and artery and tunica intima of the artery. In group III, there was weak fluorescence observed in the lumen of vein. In group IV, fluorescence was distributed principally in the lumen of the vein. In addition, fluorescence was not observed in the saphenous nerve in group I and there was mild fluorescence in the saphenous nerve in groups II, III, and IV. These findings suggest that the venous return is through "bypass route" in earlier period. In later period, the venous retrograde return is through "bypass route" and "incompetent valves route;" however, "incompetent valves route" becomes the main route. |
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Authors:
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Zhi-Zhen Jing; Shi-Min Chang; Mu-Rong You; Guang-Rong Yu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microsurgery Volume: 30 ISSN: 1098-2752 ISO Abbreviation: Microsurgery Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-11 Completed Date: 2010-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309230 Medline TA: Microsurgery Country: United States |
Other Details:
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Languages: eng Pagination: 50-4 Citation Subset: IM |
Affiliation:
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The Department of Orthopedic Surgery, Tongji Hospital, Tongji University, Shanghai, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fluorescein-5-isothiocyanate / diagnostic use* Fluorescent Dyes / diagnostic use* Hindlimb / blood supply* Microcirculation / physiology Microscopy, Fluorescence* Rabbits Regional Blood Flow / physiology Saphenous Vein* Surgical Flaps / blood supply* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 3326-32-7/Fluorescein-5-isothiocyanate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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