Document Detail


VEGF-C, VEGFR-3, and COX-2 enhances growth and metastasis of human cervical carcinoma cell lines in vitro.
MedLine Citation:
PMID:  17549374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The study was conducted to clarify the expression and biological significance of VEGF-C and VEGFR-3 in human cervical cancer cell lines and to investigate the correlation between VEGF-C and cyclooxygenase-2 (COX-2) expression in these cells. Flow cytometry, Western blotting, RT-PCR, cell immunochemistry assay, cell proliferation assay, in vitro invasion assay and cell immunochemistry assay were used to detect the gene expression and to evaluate the biological features in test cell lines. VEGF-C expression was low while VEGFR-3 was quiet high in all cell lines detected. COX-2 expression coincided with that of VEGF-C. Recombinant human VEFG-C-treated HeLa cells showed increased proliferation and invasion in a dose-depended manner while NS398, a specific inhibitor of COX-2, blocked the invasive ability of HeLa. VEGF-C and its VEGFR-3 played a crucial role in the regulation of tumor growth and metastasis in cervical cell lines, and COX-2 might be a regulator of VEGF-C expression.
Authors:
Xiaoyan Shi; Gang Chen; Hui Xing; Danhui Weng; Xiangyang Bai; Ding Ma
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  18     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-05     Completed Date:  2007-08-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  241-7     Citation Subset:  IM    
Affiliation:
Cancer Biology Research Center, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Hubei 430030, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Line, Tumor
Cell Movement
Cell Proliferation*
Cyclooxygenase 2 / genetics,  metabolism*
Female
Flow Cytometry
Humans
Membrane Proteins / genetics,  metabolism*
Neoplasm Invasiveness / pathology*
Reverse Transcriptase Polymerase Chain Reaction
Uterine Cervical Neoplasms / genetics,  metabolism*
Vascular Endothelial Growth Factor C / genetics,  metabolism*
Vascular Endothelial Growth Factor Receptor-3 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Vascular Endothelial Growth Factor C; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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