| Vectors encoding carcinoembryonic antigen fused to the B subunit of heat-labile enterotoxin elicit antigen-specific immune responses and antitumor effects. | |
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MedLine Citation:
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PMID: 18055074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vectors encoding CEA fused to the A (CEA-LTA) or B (CEA-LTB) subunits of the heat-labile enterotoxin were constructed and their immunogenicity was compared. The CEA-LTB fusion was shown to elicit a greater CEA-specific antibody and CD8+ T-cell response. Plasmid DNA and Adenovirus vectors encoding CEA-LTB proved to be immunogenic in CEA transgenic (CEA.tg) mice. CEA.tg mice immunized with repeated injections of plasmid pV1J/CEA-LTB followed by Ad/CEA-LTB were protected from tumor growth, but the adjuvant activity of the LTB protein was lost upon mutation of the LTB sequence. Depletion of T-regulatory cells increased the vaccine antitumor effect. Tumor protection was abrogated if the NK or CD8+ cell population was depleted before tumor challenge. Passive transfer studies demonstrated that CD8+ T cells contribute to the antitumor effect, thus suggesting that a genetic vaccine based on plasmid DNA and adenoviral vectors encoding CEA-LTB augments CEA-specific immune responses and significantly protects from tumor development. |
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Authors:
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Andrea Facciabene; Luigi Aurisicchio; Leonardo Elia; Fabio Palombo; Carmela Mennuni; Gennaro Ciliberto; Nicola La Monica |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-20 |
Journal Detail:
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Title: Vaccine Volume: 26 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-10 Completed Date: 2008-02-21 Revised Date: 2008-08-20 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 47-58 Citation Subset: IM |
Affiliation:
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Istituto di Ricerche di Biologia Molecolare, Via Pontina km 30,600, Pomezia 00040, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic
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genetics* Animals Bacterial Toxins / genetics*, immunology CD8-Positive T-Lymphocytes / immunology Cancer Vaccines / immunology* Carcinoembryonic Antigen / genetics*, immunology* Enterotoxins / genetics*, immunology Escherichia coli Proteins / genetics*, immunology Female Genetic Vectors Immunization Mice Mice, Inbred C57BL Mice, Transgenic Neoplasms, Experimental / therapy* Recombinant Fusion Proteins / immunology* T-Lymphocytes, Regulatory / physiology Vaccines, Synthetic / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Bacterial Toxins; 0/Cancer Vaccines; 0/Carcinoembryonic Antigen; 0/Enterotoxins; 0/Escherichia coli Proteins; 0/Recombinant Fusion Proteins; 0/Vaccines, Synthetic; 0/heat-labile enterotoxin, E coli |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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