| Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III. | |
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MedLine Citation:
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PMID: 7681830 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vaults are large cytoplasmic ribonucleoprotein particles with a sedimentation value of about 150 S. These particles contain a unique small RNA (vault RNA (vRNA)). We have determined the sequence of the RNA associated with vaults purified from both rat and bullfrog. The rat vRNA is 141 bases in length, whereas the bullfrog vRNA is present as two highly related species of 89 and 94 bases. Despite the differences in length the predicted secondary structures of the three vRNAs are clearly related. All of the vRNAs contain sequences related to the internal promoter elements necessary for transcription by RNA polymerase III. The gene for the rat vRNA was isolated and sequenced from a rat genomic library, and its transcription by RNA polymerase III was verified using an in vitro transcription assay. The rat vRNA gene was efficiently transcribed in vitro, producing a single transcript of about 140 bases. Unlike most RNA polymerase III genes, the rat vRNA is present as a single copy gene and has a distinct tissue-specific expression pattern. |
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Authors:
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V A Kickhoefer; R P Searles; N L Kedersha; M E Garber; D L Johnson; L H Rome |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 268 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-05-06 Completed Date: 1993-05-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7868-73 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, University of California School of Medicine, Los Angeles 90024-1737. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/Z11765; Z11770; Z11771 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA / genetics, isolation & purification Female Genomic Library Liver / metabolism* Models, Structural Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides Organ Specificity RNA / chemistry, genetics, metabolism* RNA Polymerase III / metabolism* Rana catesbeiana Rats Restriction Mapping Ribonucleoproteins / chemistry, metabolism* Transcription, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
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GM38097/GM/NIGMS NIH HHS; GM40623/GM/NIGMS NIH HHS; GM45299/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oligodeoxyribonucleotides; 0/Ribonucleoproteins; 63231-63-0/RNA; 9007-49-2/DNA; EC 2.7.7.-/RNA Polymerase III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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