Document Detail

Vasopressin-independent renal urinary concentration: increased rBSC1 and enhanced countercurrent multiplication.
MedLine Citation:
PMID:  12911543     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: A close association between the expression of the sodium transporter, rat bumetanide sensitive cotransporter (rBSC), in thick ascending limb of Henle and urinary concentration has been reported. However, direct evidence for this association and the mechanism of rBSC1 expression are still to be elucidated. METHODS: Brattleboro (BB) rats weighing approximately 200 g were dehydrated by water restriction for 4 hours, which induced around a 5% body weight reduction. Although plasma arginine vasopressin (AVP) was undetectable even after the water restriction, BB rats concentrated urine from 182 +/- 23 (mean +/- SD) at baseline to 404 +/- 65 mOsm/kg. H2O. RESULTS: Urinary volume was reduced from 5.8 +/- 1.8 to 1.4 +/- 0.6 mL/h. This treatment significantly increased sodium and urea accumulation in the renal medulla and reduced urinary sodium excretion. rBSC1 signals for both mRNA and protein were increased in dehydrated rats, although aquaporin type 2 (AQP2) expression was not enhanced in dehydrated BB rats. Subcutaneous infusion of desmopressin acetate (DDAVP) intensified rBSC1 signals of BB rats more than those in dehydrated condition. CONCLUSION: Dehydration increased rBSC1 expression and enhanced countercurrent multiplication even in AVP deficiency. These results supply strong evidence for the association between rBSC1 expression and urinary concentration, and indicate the presence of an AVP-independent mechanism for urine concentration.
Mari Michimata; Kazuhiko Mizukami; Michiko Suzuki; Itsuro Kazama; Yohsuke Nakamura; Katsuya Suzuki; Teruyuki Yanagisawa; Yutaka Imai; Sei Sasaki; Mitsunobu Matsubara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  64     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-12     Completed Date:  2004-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  933-8     Citation Subset:  IM    
Department of Molecular Medicine and Gene Transfer Research, Tohoku University School of Medicine, Sendai, Japan.
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MeSH Terms
Aquaporin 2
Aquaporin 6
Aquaporins / metabolism
Blood / metabolism
Electrolytes / metabolism
Kidney Concentrating Ability / physiology*
Kidney Medulla / metabolism
Rats, Brattleboro
Sodium-Potassium-Chloride Symporters / metabolism*
Urea / metabolism
Urine / chemistry
Vasopressins / physiology*
Reg. No./Substance:
0/Aqp2 protein, rat; 0/Aquaporin 2; 0/Aquaporin 6; 0/Aquaporins; 0/Electrolytes; 0/Sodium-Potassium-Chloride Symporters; 0/sodium-potassium chloride cotransporter 2 protein; 11000-17-2/Vasopressins; 57-13-6/Urea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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