Document Detail


Vasoactive intestinal polypeptide-like immunoreactivity in a human neuroblastoma cell line and the coexistence of other neuropeptide immunoreactivity in the cell line.
MedLine Citation:
PMID:  6164547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of VIP-like immunoreactivity in human neuroblastoma cell line NB-1 was demonstrated by radioimmunoassay of the crude cell extract and by immunocytochemical staining of the cells. Gel filtration profiles of VIP-like immunoreactivity in the cell extract measured by radioimmunoassays using three different region-specific antisera revealed that the immunoreactivity consists of a major molecular form corresponding to porcine VIP having 28 amino acid residues with at least two additional minor forms larger and smaller than the VIP. In addition to VIP-like immunoreactivity, the cell extract was shown to contain substance P-, neurotensin- and somatostatin-like immunoreactivity as well.
Authors:
N Yanaihara; S Kobayashi; H Sato; C Yanaihara; M Sakagami; N Sakura; T Hashimoto; S Ishikawa; T Iwanaga; T Fujita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinologia japonica     Volume:  27 Suppl 1     ISSN:  0013-7219     ISO Abbreviation:  Endocrinol. Jpn.     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-07-20     Completed Date:  1981-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376546     Medline TA:  Endocrinol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  37-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Female
Gastrointestinal Hormones / metabolism*
Humans
Neuroblastoma / analysis*
Neurotensin / analysis
Somatostatin / analysis
Substance P / analysis
Swine
Vasoactive Intestinal Peptide / metabolism*
Chemical
Reg. No./Substance:
0/Gastrointestinal Hormones; 33507-63-0/Substance P; 37221-79-7/Vasoactive Intestinal Peptide; 39379-15-2/Neurotensin; 51110-01-1/Somatostatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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