Document Detail


Vascularization of artificial beds for pancreatic islet transplantation in a rat model.
MedLine Citation:
PMID:  20692417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An alternative prevascularized bed with a subcutaneously located entrance would substantially improve islet engraftment, requiring much less invasive surgery. Studies have described times necessary for the creation of an artificial pouch suitable for subsequent islet transplantation. Polymeric mesh shaped in rounded scaffolds were implanted both subcutaneously and into the major omentum of Brown Norway female rats (n = 7). The connective tissue together with vessels were embedded into scaffolds at 1 week without regard to site. In contrast to the major omentum, vessels within the subcutaneous connective tissue surrounding the devices started to decline in 2 weeks and almost disappeared 1 week later. Magnetic resonance imaging (MRI) detected changes in fibrous tissue surrounding the wall, but only large veins located beside the devices were visible using basic MRI. The blood supply to the internal surface of the created beds was important for islet engraftment, but information could be obtained only by using dynamic contrast-enhanced MRI.
Authors:
J Kriz; D Jirak; G J Vilk; P Girman; D J White; M Hajek; F Saudek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation proceedings     Volume:  42     ISSN:  1873-2623     ISO Abbreviation:  Transplant. Proc.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-08-09     Completed Date:  2011-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2097-101     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Diabetes Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. jkri@ikem.cz
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MeSH Terms
Descriptor/Qualifier:
Analgesics, Opioid / therapeutic use
Animals
Erythrocytes / cytology
Female
Islets of Langerhans / blood supply*,  cytology
Islets of Langerhans Transplantation / methods*
Magnetic Resonance Imaging
Models, Animal
Muscle, Skeletal / cytology,  transplantation
Omentum / surgery
Pain, Postoperative / prevention & control
Prostheses and Implants
Rats
Rats, Inbred BN
Tramadol / therapeutic use
Chemical
Reg. No./Substance:
0/Analgesics, Opioid; 27203-92-5/Tramadol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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