Document Detail


Vascular smooth cell proliferation in perfusion culture of porcine carotid arteries.
MedLine Citation:
PMID:  18515073     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Objective of this study was to develop a novel in vitro artery culture system to study vascular smooth muscle cell (SMC) proliferation of porcine carotid arteries in response to injury, basic fibroblast growth factor (FGF2), and FGF2 conjugated with cytotoxin saporin (SAP). Perfusion-cultured porcine carotid arteries remained contractile in response to norepinephrine and relaxant to acetylcholine for up to 96 h. SMC proliferation of cultured arteries was detected by bromodeoxyuridine incorporation in both non-injured and balloon-injured arteries. In the inner layer of the vessel wall near the lumen, SMC proliferation were less than 10% in uninjured vessels, 66% in injured vessels, 80% in injured vessels with FGF2 treatment, and 5% in injured vessels with treatment of FGF2-SAP. Thus, the cultured porcine carotid arteries were viable; and the injury stimulated SMC proliferation, which was significantly enhanced by FGF2 and inhibited by FGF2-SAP.
Authors:
Dan Liao; Peter H Lin; Qizhi Yao; Changyi Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-06-02
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  372     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-06-25     Completed Date:  2008-07-24     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  668-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / cytology*,  drug effects,  physiology
Carotid Artery Injuries
Cell Culture Techniques*
Cell Proliferation* / drug effects
Cell Survival
Cells, Cultured
Fibroblast Growth Factor 2 / pharmacology
Muscle, Smooth, Vascular / cytology*,  drug effects,  physiology
Perfusion
Platelet-Derived Growth Factor / pharmacology
Regeneration / drug effects
Swine
Grant Support
ID/Acronym/Agency:
AT003094/AT/NCCAM NIH HHS; DE15543/DE/NIDCR NIH HHS; EB-002436/EB/NIBIB NIH HHS; HL083471/HL/NHLBI NIH HHS; HL65916/HL/NHLBI NIH HHS; HL72716/HL/NHLBI NIH HHS; R01 DE015543/DE/NIDCR NIH HHS; R01 DE015543-03/DE/NIDCR NIH HHS; R01 EB002436/EB/NIBIB NIH HHS; R01 EB002436-03/EB/NIBIB NIH HHS; R01 HL065916/HL/NHLBI NIH HHS; R01 HL065916-03/HL/NHLBI NIH HHS; R01 HL072716/HL/NHLBI NIH HHS; R01 HL072716-03/HL/NHLBI NIH HHS; R01 HL083471/HL/NHLBI NIH HHS; R01 HL083471-02/HL/NHLBI NIH HHS; R21 AT003094/AT/NCCAM NIH HHS; R21 AT003094-01/AT/NCCAM NIH HHS
Chemical
Reg. No./Substance:
0/Platelet-Derived Growth Factor; 103107-01-3/Fibroblast Growth Factor 2
Comments/Corrections

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