Document Detail

Vascular permeability factor of Pseudomonas aeruginosa.
MedLine Citation:
PMID:  4629079     Owner:  NLM     Status:  MEDLINE    
The production of vascular permeability factor (PF) by certain strains of Pseudomonas aeruginosa has been demonstrated in rabbits injected intradermally with culture filtrates followed by intravenous injection with Pontamine Sky Blue 6BX. The dose-response curve was found to be rectilinear when lesion diameters, within the range of 10 to 20 mm, were plotted against log dose. Thus, PF in test filtrates can be measured with reasonable accuracy by the concomitant testing of a reference PF. In contrast to the titers of PF obtained with Vibrio cholerae cultures, those with strains of P. aeruginosa were rather low. Thus far, PF has been demonstrated only in shallow still cultures of P. aeruginosa and not in shake cultures. A variety of commercial media were tested for the production of PF, but none was satisfactory. A synthetic medium that gave more reproducible and higher yields of PF was developed. Cultivation at 30 C generally gave higher yields of PF than at 37 C. PF was destroyed by heating at 60 C for 30 min or by digesting with trypsin or Pronase. Strains producing larger amounts of PF appeared to have greater virulence when inoculated onto the surface of burns in mice than those yielding little or no PF.
H Kusama; R H Suss
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infection and immunity     Volume:  5     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1972 Mar 
Date Detail:
Created Date:  1973-01-30     Completed Date:  1973-01-30     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  363-9     Citation Subset:  IM    
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MeSH Terms
Burns, Chemical / complications
Culture Media
Dose-Response Relationship, Drug
Lethal Dose 50
Mice, Inbred Strains
Pseudomonas Infections / complications
Pseudomonas aeruginosa / metabolism*,  pathogenicity
Serum Globulins / biosynthesis*
Vibrio cholerae / metabolism
Reg. No./Substance:
0/Culture Media; 0/Serum Globulins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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