Document Detail


Vascular endothelial growth factor-induced migration of vascular smooth muscle cells in vitro.
MedLine Citation:
PMID:  10458928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Angiogenesis is a complex process that includes recruitment and proliferation of mural cells-smooth muscle cells (SMC) and pericytes. Vascular endothelial growth factor (VEGF) has been shown to play an important role in angiogenesis and is an endothelial cell chemoattractant. In addition, certain VEGF isoforms have been implicated in the normal formation of smooth muscle cell-surrounded arteries. Because VEGF's role as a mural cell chemoattractant had not been explored, we examined the ability of VEGF to influence vascular SMC migration in vitro. A Boyden chamber migration assay demonstrated that VEGF (0-100 ng/ml) caused a dose-dependent migration of SMC. VEGF did not cause proliferation of SMC. Reverse transcriptase-polymerase chain reaction analysis demonstrated the presence of both KDR and flt mRNA, two known VEGF receptors, in SMC cultures. Western blot analysis of SMC lysates confirmed these data, revealing bands migrating at approximately 200 kDa and slightly below 200 kDa consistent with KDR and flt. These observations demonstrate that VEGF receptors are present on SMC, and that VEGF can act as an SMC chemoattractant.
Authors:
C L Grosskreutz; B Anand-Apte; C Dupláa; T P Quinn; B I Terman; B Zetter; P A D'Amore
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microvascular research     Volume:  58     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-18     Completed Date:  1999-10-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  128-36     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Department of Surgery, Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cattle
Cell Movement / drug effects
Cells, Cultured
DNA Primers / genetics
Endothelial Growth Factors / pharmacology*,  physiology
Endothelium, Vascular / cytology,  drug effects,  physiology
Humans
Lymphokines / pharmacology*,  physiology
Muscle, Smooth, Vascular / cytology*,  drug effects*,  physiology
Neovascularization, Physiologic / drug effects
Proto-Oncogene Proteins / drug effects,  genetics,  physiology
Receptor Protein-Tyrosine Kinases / drug effects,  genetics,  physiology
Receptors, Growth Factor / drug effects,  genetics,  physiology
Receptors, Vascular Endothelial Growth Factor
Recombinant Proteins / pharmacology
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factors
Grant Support
ID/Acronym/Agency:
K11-EY00342/EY/NEI NIH HHS; R01EY05318/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Endothelial Growth Factors; 0/Lymphokines; 0/Proto-Oncogene Proteins; 0/Receptors, Growth Factor; 0/Recombinant Proteins; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.1/Receptors, Vascular Endothelial Growth Factor; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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