| Vascular chemical sulfhydryl alkylation in vitro: alterations in intracellular calcium and cAMP and cGMP metabolism. | |
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MedLine Citation:
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PMID: 9151287 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined the effect of N-ethylmaleimide (NEM, 10(-7) M) on agonist-induced contraction and the relaxation following drug-washout, of vascular smooth muscle (VSM) segments derived from hypertensive rabbits. Mean blood pressure increase was produced either by renal constriction plus contralateral nephrectomy, or by cadmium acetate ingestion. Freely-ionized calcium (45Ca)flux, cyclic 3':5'-guanosine monophosphate (cGMP), and cyclic 3':5' adenosine monophosphate (cAMP), were analyzed. NEM was used as a stereoselective probe to clarify the role of sulfhydryl (SH) groups in hypertension. Contractile response to norepinephrine (NE, 5.9 x 10(-7) M), angiotensin II (AT, 9.8 x 10(-8) M), and potassium chloride (KCl, 2.2 x 10(-2) M) were significantly depressed in hypertensive tissue. Exposure to NEM, before agonist challenge, caused an even greater depression in contractile response. As for the normotensive group, an inhibition of relaxation occurred when NEM was added after the development of a maximal contractile response to NE, AT or KCl. Changes in contractile ability and in relaxation were attributed to specific alterations in calcium distribution. These alterations were examined by 45Ca washout components and were related to cAMP and cGMP metabolism. These results suggest a regulatory role of SH groups in contraction and relaxation and a modification of this role in hypertension. |
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Authors:
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J F Tomera |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods and findings in experimental and clinical pharmacology Volume: 19 ISSN: 0379-0355 ISO Abbreviation: Methods Find Exp Clin Pharmacol Publication Date: 1997 Mar |
Date Detail:
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Created Date: 1997-07-22 Completed Date: 1997-07-22 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7909595 Medline TA: Methods Find Exp Clin Pharmacol Country: SPAIN |
Other Details:
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Languages: eng Pagination: 113-24 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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toxicity Alkylation Angiotensin II / pharmacokinetics Animals Cadmium / toxicity Calcium / metabolism* Cyclic AMP / metabolism* Cyclic GMP / metabolism* Ethylmaleimide / pharmacology* Female Half-Life Hypertension, Renal / chemically induced, metabolism* Muscle, Smooth, Vascular / drug effects*, metabolism Norepinephrine / pharmacokinetics Potassium Chloride / pharmacokinetics Rabbits Sulfhydryl Reagents / pharmacology* Vasoconstriction / drug effects Vasodilation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Sulfhydryl Reagents; 11128-99-7/Angiotensin II; 128-53-0/Ethylmaleimide; 51-41-2/Norepinephrine; 543-90-8/cadmium acetate; 60-92-4/Cyclic AMP; 7440-43-9/Cadmium; 7440-70-2/Calcium; 7447-40-7/Potassium Chloride; 7665-99-8/Cyclic GMP |
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