| Vascular activity of polycations and basic amino acids: L-arginine does not specifically elicit endothelium-dependent relaxation. | |
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MedLine Citation:
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PMID: 2463834 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Irrespective of their stereochemistry (D- or L-form), polycations such as poly-lysine, poly-arginine and poly-histidine elicited endothelium dependent relaxation of pre-contracted rat aortic rings in a dose-dependent manner (ED50 less than or equal to 10-7 M). In contrast, the basic amino acids arginine, glutamine, histidine and lysine caused only endothelium-potentiated relaxation at high concentrations (ED 50 greater than 10-3 M). Both heparin (1U/ml) and dextran sulphate (10 microgram/ml) abolished relaxation by the polycations but had no effect on the responses to the basic amino acids or acetylcholine. These results indicate that the vasodilatory property of the polycations is due to an electrostatic interaction with anionic domains on the endothelial surface, whereas the basic amino acids elicit a non-specific relaxation. Therefore, L-arginine per se cannot be the immediate precursor of nitric oxide, the proposed endothelium-derived relaxing factor. |
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Authors:
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G Thomas; M Hecker; P W Ramwell |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 158 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-02-17 Completed Date: 1989-02-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 177-80 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, D.C. 20007 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Amino Acids, Diamino / pharmacology* Animals Aorta Arginine / analogs & derivatives, pharmacology* Dextran Sulfate Dextrans / pharmacology Dose-Response Relationship, Drug Endothelium, Vascular / drug effects* Heparin / pharmacology Male Muscle Relaxation / drug effects Muscle, Smooth, Vascular / drug effects* Rats Rats, Inbred Strains Vasodilation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Diamino; 51-84-3/Acetylcholine; 74-79-3/Arginine; 9004-54-0/Dextrans; 9005-49-6/Heparin; 9042-14-2/Dextran Sulfate; 971-21-1/benzoylarginine ethyl ester |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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