Document Detail


Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.
MedLine Citation:
PMID:  23140521     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: We sought to determine some of the molecular requirements for basal state "tone" of skeletal muscle arterioles in vivo, and whether asynchronous Ca(2+) waves are involved or not.
METHODS: Cremaster muscles of anesthetized exMLCK and smGCaMP2 biosensor mice were exteriorized, and the fluorescent arterioles were visualized with wide-field, confocal or multiphoton microscopy to observe Ca(2+) signaling and arteriolar diameter.
RESULTS: Basal state tone of the arterioles was ~50%. Local block of Ang-II receptors (AT1 ) or α1 -adrenoceptors (α1 -AR) had no effect on diameter, nor did complete block of sympathetic nerve activity (SNA). Inhibition of phospholipase C caused dilation nearly to the Ca(2+) -free (passive) diameter, as did exposure to nifedipine or 2-APB. Arterioles were also dilated when treated with SKF96365. High-resolution imaging of exMLCK fluorescence (ratio) or GCaMP2 fluorescence in smooth muscle cells failed to reveal Ca(2+) waves (although Ca(2+) waves/transients were readily detected by both biosensors in small arteries, ex vivo).
CONCLUSIONS: Arterioles of cremaster muscle have vascular tone of ~ 50%, which is not due to α1 -AR, AT1 R, or SNA. PLC activity, L-type Ca(2+) channels, 2-APB- and SKF96365-sensitive channels are required. Propagating Ca(2+) waves are not present. A key role for PLC and InsP3 R in vascular tone in vivo, other than producing Ca(2+) waves, is suggested.
Authors:
Joseph R H Mauban; Joseph Zacharia; Jin Zhang; Withrow Gil Wier
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  20     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-11     Completed Date:  2013-11-01     Revised Date:  2014-05-14    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-77     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arterioles / cytology,  metabolism
Calcium / metabolism*
Calcium Channels / metabolism
Calcium Signaling / physiology*
Mice
Mice, Transgenic
Microscopy, Fluorescence, Multiphoton
Muscle, Skeletal / blood supply*,  cytology
Grant Support
ID/Acronym/Agency:
1 R01HL 107654/HL/NHLBI NIH HHS; 1R01 HL091969/HL/NHLBI NIH HHS; R01 DK077140/DK/NIDDK NIH HHS; R01 HL091969/HL/NHLBI NIH HHS; R01 HL107654/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channels; SY7Q814VUP/Calcium
Comments/Corrections

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