Document Detail


Vascular leak is a central feature in the pathogenesis of systemic sclerosis.
MedLine Citation:
PMID:  22660809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The main histopathological focus of systemic sclerosis (SSc) has concentrated on fibrotic changes. We investigated the microvasculature alterations in the skin of patients with SSc at various stages of disease duration with whole-field digital microscopy.
METHODS: Twenty consecutive patients with SSc, 1 with Raynaud's phenomenon (RP) without SSc, and 4 healthy controls underwent punch biopsy on the medial forearm. Eighteen patients were included in the primary analysis. Two with recent-onset diffuse cutaneous disease, 1 repeat SSc biopsy, and 1 patient with RP without SSc were also evaluated. All specimens were processed with histochemical stains and immunohistochemistry. We analyzed microvasculature abnormalities in an objective and systematic manner taking advantage of recent advances in whole-field digital microscopy. This analysis was coupled with ultrastructural evaluation performed with transmission electron microscopy (TEM).
RESULTS: Whole-field digital microscopy and TEM of SSc skin biopsies revealed that endothelial abnormalities are a universal feature regardless of clinical features and/or duration of disease. These features were not seen in any healthy control specimens or in the single RP patient samples. Whole-field digital microscopy identified increased interstitial edema (31.0% ± 9.6% vs 17.6% ± 3.3% in controls; p = 0.009) and fibrosis (75.6% ± 5.7% vs 66.1% ± 9.8% in controls; p = 0.02) in all patients with SSc. Lower CD34 staining was seen in SSc compared to healthy controls (0.32% ± 0.22% vs 1.31% ± 0.34%; p < 0.0001) and within the SSc population with interstitial lung disease (0.55% ± 0.22% vs 0.15% ± 0.16%; p = 0.01). Perivascular and interstitial infiltrate of mast cells was present in all SSc specimens.
CONCLUSION: Whole-field digital microscopy offers a means of rapidly carrying out objective, fully quantitative, and reproducible measurements of microscopic features of SSc microvascular change. The universal morphologically abnormal endothelial cells and interstitial edema in all patients with SSc biopsied suggests that SSc may be intrinsically a disease of the endothelium characterized by vascular leak.
Authors:
Tracy M Frech; Monica P Revelo; Stavros G Drakos; Maureen A Murtaugh; Boaz A Markewitz; Allen D Sawitzke; Dean Y Li
Related Documents :
19046909 - Lack of effect of igf-i on the glomerular filtration rate in non-diabetic patients with...
21253869 - α-2-hs-glycoprotein is a potential marker predicting hepatitis b e antigen seroconvers...
21296899 - The perfect crime? ccsvi not leaving a trace in ms.
21402389 - Oromandibular dystonia in encephalitis.
3588249 - Influence of protein binding and severity of illness on renal elimination of four cepha...
10078779 - Influence of fever on cefazolin pharmacokinetics.
19744179 - Clinical and laboratory study of rosacea in northern greece.
23857459 - Comparative study of the safety of regadenoson between patients with mild/moderate chro...
15997419 - The csf levels of total-tau and phosphotau in patients with relapsing-remitting multipl...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-06-01
Journal Detail:
Title:  The Journal of rheumatology     Volume:  39     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-11-26     Revised Date:  2013-07-08    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1385-91     Citation Subset:  IM    
Affiliation:
Division of Rheumatology, Department of Internal Medicine, University of Utah, 4B200 SOM, 30 N 1900 E, Salt Lake City, Utah 84132, USA.. tracy.frech@hsc.utah.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antigens, CD34 / analysis
Capillary Leak Syndrome / pathology*
Edema / pathology
Endothelium, Vascular / pathology*,  ultrastructure
Female
Humans
Lung Diseases, Interstitial / pathology
Male
Mast Cells
Microscopy
Microvessels
Middle Aged
Raynaud Disease / pathology
Scleroderma, Systemic / pathology*
Severity of Illness Index
Skin / blood supply*,  pathology
Grant Support
ID/Acronym/Agency:
R01 HL077671/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD34
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Preventing friction-induced chondrocyte apoptosis: comparison of human synovial fluid and hylan G-F ...
Next Document:  Colchicine use is associated with decreased prevalence of myocardial infarction in patients with gou...