Document Detail


Vascular Endothelial (VEGF) and Epithelial Growth Factor (EGF) as Well as Platelet-Activating Factor (PAF) and Receptors are Expressed in the Early Pregnant Canine Uterus.
MedLine Citation:
PMID:  22458960     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of this study was to investigate the course of expression of platelet-activating factor (PAF), PAF-receptor (PAF-R), epidermal growth factor (EGF), EGF-R, vascular endothelial growth factor (VEGF), VEGF-R1 and VEGF-R2 in uterine tissue during canine pregnancy. For this purpose, 20 bitches were ovariohysterectomized at days 10-12 (n = 10), 18-25 (n = 5) and 28-45 (n = 5) days after mating, respectively. The pre-implantation group was proven pregnant by embryo flushing of the uterus after the operation, the others by sonography. Five embryo negative, that is, non-pregnant, bitches in diestrus (day 10-12) served as controls. Tissue samples from the uterus (placentation sites and horn width, respectively) were excised and snap-frozen in liquid nitrogen after embedding in Tissue Tec(®) . Extraction of mRNA for RT-PCR was performed with Tri-Reagent. In the embryos, mRNA from all factors except VEGF was detected. In the course of pregnancy, significantly higher expression of PAF and PAFR as well as VEGF and VEGFR2 during the pre-implantation stage than in all other stages and a strong upregulation of EGF during implantation were characteristic. The course of EGF was in diametrical opposition to the course of the receptor. These results point towards an increased demand for VEGF, EGF and PAF during the earliest stages of canine pregnancy.
Authors:
S Schäfer-Somi; S Sabitzer; D Klein; E Reinbacher; H Kanca; Hb Beceriklisoy; Oa Aksoy; I Kucukaslan; Hc Macun; S Aslan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-28
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  -     ISSN:  1439-0531     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Verlag GmbH.
Affiliation:
Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Vienna, Austria VetCORE - Facility for Research, University of Veterinary Medicine Vienna, Vienna, Austria Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, University of Ankara, Diskapi, Ankara, Turkey Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, University of Kirikkale, Yahsihan, Kirikkale, Turkey.
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