Document Detail


Vascular changes in the corpus luteum during early pregnancy in the cow.
MedLine Citation:
PMID:  20103987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study determined vascular changes in the bovine corpus luteum (CL) at Day 16 (early maternal recognition period) and Day 40 in early pregnancy and compared them to the CL from Day 12 and Day 16 of the estrous cycle. The CLs were analyzed in the central and peripheral regions, where site-depending features of vessels and angiogenic factors are evident. The same protein level of the endothelial cell marker von Willebrand factor was retained in the CL from Day 16 of the estrous cycle to Day 40 of early pregnancy. The protein level of pericytes and smooth muscle cells was determined using smooth muscle alpha-actin; the level decreased at Day 40 of early pregnancy in both regions of the CL. No significant change in the expressions of vascular endothelial growth factors VEGF(164) and VEGF(120) mRNA occurred from Day 16 of the estrous cycle until Day 40 of early pregnancy. Angiopoietin (ANGPT)-2 / ANGPT-1 mRNA ratio (an index of instability of vasculature) increased in the periphery at Day 16 of the estrous cycle and then decreased until Day 40 of early pregnancy. The results suggest that there is no difference in vascular structure between non-pregnant and pregnant luteal tissue during the early maternal recognition period (Day 16). Also, luteal rescue by early pregnancy may be not associated with further blood vessel formation but rather may be related to the decrease of blood vessels per unit of area and blood vessel stabilization in the bovine CL.
Authors:
Nicola Beindorff; Kaya Nagai; Koumei Shirasuna; Kathrin Herzog; Katharina Hoeffmann; Motoki Sasaki; Heinrich Bollwein; Akio Miyamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-27
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  56     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  263-70     Citation Subset:  IM    
Affiliation:
Clinic for Cattle, University of Veterinary Medicine Hannover, Hanover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Angiopoietin-1 / genetics
Angiopoietin-2 / genetics
Animals
Cattle / physiology*
Corpus Luteum / blood supply*,  cytology*
Endothelial Cells / physiology
Estrous Cycle / physiology*
Female
Myocytes, Smooth Muscle / physiology
Pericytes / physiology
Pregnancy
Pregnancy, Animal / physiology*
Progesterone / blood
RNA, Messenger / metabolism
Receptor, TIE-2 / genetics
Vascular Endothelial Growth Factor A / genetics
Chemical
Reg. No./Substance:
0/Angiopoietin-1; 0/Angiopoietin-2; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 57-83-0/Progesterone; EC 2.7.10.1/Receptor, TIE-2

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