Document Detail

Vascular cell kinetics in response to intimal injury ex vivo.
MedLine Citation:
PMID:  19672106     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recent studies indicate that the smooth muscle-like cells contributing to neointimal hyperplasia after vascular injury derive from circulating precursor cells. Here, we define the time course of precursor cell influx, the roles of separate vascular layers, and the relative role of migration versus proliferation to intimal hyperplasia. METHODS AND RESULTS: After rat aortic denudation injury the neointimal cell number increased several 100-fold between days 4 and 28, preceded by a 5-fold increase in the number of adventitial cells and a 4-fold increase in the number of adventitial microvessels. The influx, migration, and maturation of neointimal cells were quantitated by culturing whole vessel explants at different time points after injury. Explant outgrowth increased 14-fold, and cell migration 3.5-fold on days 2-14 after injury. Cell proliferation increased less than 2-fold. The frequency of precursors to outgrowing cells, determined using limiting dilution analysis, increased 8-fold between days 2 and 4 after injury. Many outgrowing cells displayed characteristics of undifferentiated cells. CONCLUSIONS: Adventitial activation precedes development of the neointima, and precursor cell influx occurs on days 2-14 after injury. Cell migration, more than proliferation, contributes to fibrointimal dysplasia. These findings underline the importance of early therapeutic intervention with antimigratory compounds to prevent neointimal hyperplasia.
Nina-Maria Tigerstedt; Hanna Savolainen-Peltonen; Satu Lehti; Pekka Hayry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-06
Journal Detail:
Title:  Journal of vascular research     Volume:  47     ISSN:  1423-0135     ISO Abbreviation:  J. Vasc. Res.     Publication Date:  2010  
Date Detail:
Created Date:  2009-12-09     Completed Date:  2009-12-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  35-44     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Transplantation Laboratory, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
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MeSH Terms
Aorta, Thoracic / injuries,  metabolism,  pathology*
Biological Markers / metabolism
Cell Differentiation
Cell Movement
Cell Proliferation*
Connective Tissue / blood supply
Microvessels / pathology
Myocytes, Smooth Muscle / metabolism,  pathology*
Organ Culture Techniques
Stem Cells / metabolism,  pathology*
Tunica Intima / injuries,  metabolism,  pathology*
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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