Document Detail

Various models of cardiac conditioning in single or sequential periods of ischemia: Comparative effects on infarct size and intracellular signaling.
MedLine Citation:
PMID:  23270747     Owner:  NLM     Status:  Publisher    
BACKGROUND: Preconditioning (PreC) and postconditioning (PostC) reduce infarct size. We sought to determine the effects of PreC and PostC, alone or in combination, on infarct size and expression of intracellular signals in different ischemia models. METHODS: Male rabbits were subjected to myocardial ischemia followed by 3-hour reperfusion. In a first series we applied 3 ischemia models [a 20-min period (20), a 40-min period (40), and two sequential 20-min periods (20-20)] and 3 types of interventions [no intervention (controls, C), 2cycles of 5-min ischemia/10-min reperfusion before index ischemia (PreC) and 6cycles of 10-s ischemia/10-s reperfusion after index ischemia and/or between the sequential ischemic periods (PostC)] (12 groups in total). Infarct size (I) and area at risk (R) were assessed (%I/R). In a second series, samples were taken for western blot analysis of Akt phosphorylation. RESULTS: Overall, %I/R differed significantly among groups (p<0.001). In control groups, C-40 had a greater %I/R than C-20 (p=0.006). In intervention groups, no differences were found in %I/R. All intervention groups had significantly lower %I/R compared to C-40 group (p<0.001), whereas, compared to C-20-20 group, PreC-20-20, 20-PostC-20, 20-PostC-20-PostC and PreC-20-20-PostC groups had lower %I/R (all p<0.05). Akt was increased in all groups in which a significant %I/R reduction was achieved (p<0.05 versus all other groups). CONCLUSIONS: PreC and PostC, alone or in combination, are effective when an ischemic insult of a given duration is applied either as a single or as sequential periods. Protection from either intervention is associated with an enhanced Akt activation.
Konstantinos Iliodromitis; Dimitrios Farmakis; Ioanna Andreadou; Anastasia Zoga; Sofia-Iris Bibli; Theodora Manolaki; Nikolaos Dagres; Efstathios K Iliodromitis; Maria Anastasiou-Nana; Dimitrios Th Kremastinos
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-24
Journal Detail:
Title:  International journal of cardiology     Volume:  -     ISSN:  1874-1754     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
Second Department of Cardiology, University of Athens Medical School, Attikon University Hospital, Athens, Greece.
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