| Varicella-zoster virus-induced apoptosis in MeWo cells is accompanied by down-regulation of Bcl-2 expression. | |
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MedLine Citation:
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PMID: 20345323 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Varicella-zoster virus infects multiple human and monkey cells in culture. The mode of cell death appears to be autophagy or apoptosis. Analysis of VZV-infected human melanoma (MeWo) cells revealed that Bcl-2 mRNA and protein levels were decreased significantly 64 and 72 hpi (hours post infection), accompanied by the release of cytochrome c from mitochondria into the cytoplasm. Western blot analysis of virus-infected cells revealed activation of caspase-8, a marker for the extrinsic pathway of apoptosis, and caspase-9, a marker for the intrinsic pathway of apoptosis at 64 and 72 hpi. Significant increases in the levels of cleaved caspase-3 and cleaved poly (ADP) ribose polymerase (PARP) were also seen at the height of cytopathic effect. Thus VZV induces apoptosis in MeWo cells in which Bcl-2 is down-regulated. Future studies will determine differences in the cascade of apoptotic events in non-neuronal cells compared to neurons that allow VZV to become latent. |
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Authors:
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Elizabeth Brazeau; Ravi Mahalingam; Don Gilden; Mary Wellish; Benedikt B Kaufer; Nikolaus Osterrieder; Subbiah Pugazhenthi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of neurovirology Volume: 16 ISSN: 1538-2443 ISO Abbreviation: J. Neurovirol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-05-06 Completed Date: 2010-12-02 Revised Date: 2011-06-16 |
Medline Journal Info:
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Nlm Unique ID: 9508123 Medline TA: J Neurovirol Country: England |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Affiliation:
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Department of Neurology. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology* Blotting, Western Cell Line, Tumor Down-Regulation Herpesvirus 3, Human / metabolism* Humans Proto-Oncogene Proteins c-bcl-2 / biosynthesis* Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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AG006127/AG/NIA NIH HHS; AG032958/AG/NIA NIH HHS; NS007321/NS/NINDS NIH HHS; NS032623/NS/NINDS NIH HHS; P01 AG032958-03/AG/NIA NIH HHS; P01 NS032623-20/NS/NINDS NIH HHS; R01 AG006127-25/AG/NIA NIH HHS; R37 AG006127-18/AG/NIA NIH HHS; T32 NS007321-20/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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