Document Detail


Variations in amplification and expression of the ornithine decarboxylase gene in human breast cancer cells.
MedLine Citation:
PMID:  1663805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polyamine biosynthetic pathway plays a critical role in the growth of human breast cancer cells. Ornithine decarboxylase (ODC) is a key enzyme in polyamine biosynthesis. To understand the regulation of ODC activity and polyamine accumulation in breast cancer cells, we studied amplification and expression of the ODC gene in four breast cancer cell lines. ODC gene dosage was analyzed by Southern blot hybridization and was 4- to 12-fold higher in T-47D, MDA-MB-231, and BT-20 cell lines than in the MCF-7 cell line. ODC mRNA level was 2- to 3-fold higher in BT-20 and MDA-MB-231 cell lines than in the other two lines. We also measured ODC activity and polyamine concentration in these cell lines, and determined their sensitivity to an ODC inhibitor, difluoromethylornithine (DFMO). BT-20 cells showed significantly higher ODC activity and polyamine concentrations than the other three cell lines. BT-20 cells were resistant to the growth inhibitory effect of DFMO even at 4 mM concentration, whereas the proliferation of MCF-7, T47D, and MDA-MB-231 cells was inhibited by this drug. These results suggest that different transcriptional and post-transcriptional mechanisms control the regulation of ODC gene expression in breast cancer cell lines.
Authors:
T Thomas; D T Kiang; O A Jänne; T J Thomas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Breast cancer research and treatment     Volume:  19     ISSN:  0167-6806     ISO Abbreviation:  Breast Cancer Res. Treat.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-03-12     Completed Date:  1992-03-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8111104     Medline TA:  Breast Cancer Res Treat     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  257-67     Citation Subset:  IM    
Affiliation:
Department of Environmental and Community Medicine, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / enzymology,  pathology*
Breast Neoplasms / enzymology,  pathology*
Carcinoma / enzymology,  pathology*
Carcinoma, Intraductal, Noninfiltrating / enzymology,  pathology*
DNA, Neoplasm / genetics
Eflornithine / pharmacology
Enzyme Induction
Estrogens
Female
Gene Amplification
Gene Expression Regulation, Neoplastic
Humans
Neoplasm Proteins / biosynthesis,  genetics*
Neoplasms, Hormone-Dependent / enzymology,  pathology
Ornithine Decarboxylase / biosynthesis,  genetics*
Polyamines / analysis
RNA, Messenger / biosynthesis
RNA, Neoplasm / analysis
Tumor Cells, Cultured / drug effects,  enzymology
Grant Support
ID/Acronym/Agency:
CA42439/CA/NCI NIH HHS; CA51487/CA/NCI NIH HHS; HD13541/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Estrogens; 0/Neoplasm Proteins; 0/Polyamines; 0/RNA, Messenger; 0/RNA, Neoplasm; 70052-12-9/Eflornithine; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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