| Variations in amplification and expression of the ornithine decarboxylase gene in human breast cancer cells. | |
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MedLine Citation:
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PMID: 1663805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polyamine biosynthetic pathway plays a critical role in the growth of human breast cancer cells. Ornithine decarboxylase (ODC) is a key enzyme in polyamine biosynthesis. To understand the regulation of ODC activity and polyamine accumulation in breast cancer cells, we studied amplification and expression of the ODC gene in four breast cancer cell lines. ODC gene dosage was analyzed by Southern blot hybridization and was 4- to 12-fold higher in T-47D, MDA-MB-231, and BT-20 cell lines than in the MCF-7 cell line. ODC mRNA level was 2- to 3-fold higher in BT-20 and MDA-MB-231 cell lines than in the other two lines. We also measured ODC activity and polyamine concentration in these cell lines, and determined their sensitivity to an ODC inhibitor, difluoromethylornithine (DFMO). BT-20 cells showed significantly higher ODC activity and polyamine concentrations than the other three cell lines. BT-20 cells were resistant to the growth inhibitory effect of DFMO even at 4 mM concentration, whereas the proliferation of MCF-7, T47D, and MDA-MB-231 cells was inhibited by this drug. These results suggest that different transcriptional and post-transcriptional mechanisms control the regulation of ODC gene expression in breast cancer cell lines. |
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Authors:
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T Thomas; D T Kiang; O A Jänne; T J Thomas |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Breast cancer research and treatment Volume: 19 ISSN: 0167-6806 ISO Abbreviation: Breast Cancer Res. Treat. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-03-12 Completed Date: 1992-03-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8111104 Medline TA: Breast Cancer Res Treat Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 257-67 Citation Subset: IM |
Affiliation:
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Department of Environmental and Community Medicine, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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enzymology,
pathology* Breast Neoplasms / enzymology, pathology* Carcinoma / enzymology, pathology* Carcinoma, Intraductal, Noninfiltrating / enzymology, pathology* DNA, Neoplasm / genetics Eflornithine / pharmacology Enzyme Induction Estrogens Female Gene Amplification Gene Expression Regulation, Neoplastic Humans Neoplasm Proteins / biosynthesis, genetics* Neoplasms, Hormone-Dependent / enzymology, pathology Ornithine Decarboxylase / biosynthesis, genetics* Polyamines / analysis RNA, Messenger / biosynthesis RNA, Neoplasm / analysis Tumor Cells, Cultured / drug effects, enzymology |
| Grant Support | |
ID/Acronym/Agency:
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CA42439/CA/NCI NIH HHS; CA51487/CA/NCI NIH HHS; HD13541/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Estrogens; 0/Neoplasm Proteins; 0/Polyamines; 0/RNA, Messenger; 0/RNA, Neoplasm; 70052-12-9/Eflornithine; EC 4.1.1.17/Ornithine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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