| Variations in surgical procedures for hind limb ischaemia mouse models result in differences in collateral formation. | |
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MedLine Citation:
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PMID: 20705493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To identify the optimal mouse model for hind limb ischaemia, which offers a therapeutic window that is large enough to detect improvements of blood flow recovery, for example, using cell therapies. MATERIALS AND METHODS: Different surgical approaches were performed: single coagulation of femoral and iliac artery, total excision of femoral artery and double coagulation of femoral and iliac artery. Blood flow restoration was analysed with laser Doppler perfusion imaging (LDPI). Immuno-histochemical stainings, angiography and micro-computed tomography (CT) scans were performed for visualisation of collaterals in the mouse. RESULTS: Significant differences in flow restoration were observed depending on the surgical procedure. After single coagulation, blood flow already restored 100% in 7 days, in contrast to a significant delayed flow restoration after double coagulation (54% after 28 days, P<0.001). After total excision, blood flow was 100% recovered within 28 days. Compared with total excision, double coagulation displayed more pronounced corkscrew phenotype of the vessels typical for collateral arteries on angiographs. CONCLUSION: The extent of the arterial injury is associated with different patterns of perfusion restoration. The double coagulation mouse model is, in our hands, the best model for studying new therapeutic approaches as it offers a therapeutic window in which improvements can be monitored efficiently. |
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Authors:
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A A Hellingman; A J N M Bastiaansen; M R de Vries; L Seghers; M A Lijkwan; C W Löwik; J F Hamming; P H A Quax |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-12 |
Journal Detail:
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Title: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery Volume: 40 ISSN: 1532-2165 ISO Abbreviation: Eur J Vasc Endovasc Surg Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-01-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9512728 Medline TA: Eur J Vasc Endovasc Surg Country: England |
Other Details:
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Languages: eng Pagination: 796-803 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Vascular Surgery, Leiden University Medical Center, Leiden, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Flow Velocity Collateral Circulation* Disease Models, Animal Electrocoagulation* Femoral Artery / physiopathology, radiography, surgery*, ultrasonography Hindlimb Iliac Artery / physiopathology, radiography, surgery*, ultrasonography Immunohistochemistry Interleukin Receptor Common gamma Subunit / deficiency, genetics Ischemia / diagnosis, etiology, physiopathology* Laser-Doppler Flowmetry Male Mice Mice, Inbred C57BL Mice, Inbred NOD Mice, Knockout Mice, SCID Muscle, Skeletal / blood supply* Regional Blood Flow Time Factors Vascular Surgical Procedures* X-Ray Microtomography |
| Chemical | |
Reg. No./Substance:
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0/Il2rg protein, mouse; 0/Interleukin Receptor Common gamma Subunit |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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