Document Detail


Variations in Jun and Fos protein expression and AP-1 activity in cycling, resting and stimulated fibroblasts.
MedLine Citation:
PMID:  9047389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have analysed the different Jun and Fos proteins as NIH3T3 fibroblasts pass from exponential growth to quiescence and during the first 24 h after their re-entry into the cell cycle following serum stimulation. We show that these proteins can be divided into 3 subgroups based on their pattern of expression. The first contains c-Jun, Jun-D and Fra-2 which are expressed at high level in cycling cells and are only mildly induced by serum. The second contains Jun-B, c-Fos, Fos-B and deltaFos-B whose levels are low in cycling cells but increase strongly and rapidly after stimulation by serum. The third group contains only Fra-1, which is absent from cycling cells and behaves as a delayed early response protein after serum stimulation. AP-1 binding activity is low both in cycling and quiescent fibroblasts but increases after stimulation by serum with kinetics matching the induction of the various Jun and Fos proteins. Antibody supershift analyses demonstrate that the composition of AP-1 binding activity reflects the relative abundance of each Jun and Fos protein. Furthermore, the state of post-translational modification varies continuously for all of the AP-1 proteins as growth conditions change. These data indicate that AP-1 activity during the G0-G1 transition is finely regulated and complex, involving changes both in protein expression and in posttranslational modification.
Authors:
D Lallemand; G Spyrou; M Yaniv; C M Pfarr
Related Documents :
25169209 - Brain metastasis is predetermined in early-stages of cutaneous melanoma by cd44v6 expre...
25297929 - A clinicopathological analysis of primary mucosal malignant melanoma.
7850019 - Dynamic changes in the composition of the ap-1 transcription factor dna-binding activit...
2176599 - Neurokinin a induces expression of the c-fos, c-jun, and c-myc genes in rat smooth musc...
23872359 - Mirna and mrna expression profiling identifies members of the mir-200 family as potenti...
1321269 - Epstein-barr virus bzlf1 transactivator is a negative regulator of jun.
11585639 - Upregulation of the inhibitor of apoptosis proteins in activated t lymphocytes from pat...
19759179 - Expression and activity of phosphodiesterase isoforms during epithelial mesenchymal tra...
19285009 - Identification and characterization of stem cell-specific transcription of psf1 in sper...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  14     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  819-30     Citation Subset:  IM    
Affiliation:
Unité des Virus Oncogènes, UA 1149 du CNRS Département des Biotechnologies, Institut Pasteur, Paris, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Antibody Specificity
Cell Cycle
DNA / metabolism
Mice
Molecular Weight
Phosphorylation
Proto-Oncogene Proteins c-fos / analysis*,  metabolism
Proto-Oncogene Proteins c-jun / analysis*,  metabolism
Transcription Factor AP-1 / metabolism*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Alterations of the p16 gene in head and neck cancer: frequency and association with p53, PRAD-1 and ...
Next Document:  Identification of a dominant-negative mutation in the yeast CDC25 guanine nucleotide exchange factor...