Document Detail

Variations and distributions of O-glycosidically linked sugar chains in bovine kappa-casein.
MedLine Citation:
PMID:  1500573     Owner:  NLM     Status:  MEDLINE    
The variation and distribution of O-glycosidically linked sugar chains in kappa-casein from bovine mature milk were analyzed by HPLC, fast atom bombardment mass spectrometry, and 13C nuclear magnetic resonance. A monosaccharide alditol (N-acetylgalactosaminitol) and four oligosaccharide alditols [a neutral disaccharide (galactosyl-beta 1-3N-acetylgalactosaminitol), two acidic trisaccharides (straight chain: N-acetylneuraminyl alpha 2-3galactosyl-beta 1-3N-acetylgalactosaminitol and branch chain: galactosyl beta 1-3(N-acetylneuraminyl alpha 2-6)N-acetylgalactosaminitol), and an acidic tetrasaccharide(N-acetylneuraminyl alpha 2-3galactosyl-beta 1-3(N-acetylgalactosaminyl alpha 2-6)N-acetylgalactosaminitol] were identified as sugar chains linking on normal bovine kappa-casein. The most dominant sugar chain was an acidic tetrasaccharide. The four oligosaccharide chains were identical to those already found, but N-acetylgalactosamine was a newly identified sugar chain linking on kappa-casein. The distribution of monosaccharide, disaccharide, trisaccharide (straight), trisaccharide (branched), and tetrasaccharide chains were determined by HPLC to be .8, 6.3, 18.4, 18.5, and 56.0%, respectively (means of five kappa-caseins).
T Saito; T Itoh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  75     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-09-16     Completed Date:  1992-09-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1768-74     Citation Subset:  IM    
Laboratory of Animal Products Chemistry, College of Agriculture, Tohoku University, Miyagi, Japan.
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MeSH Terms
Carbohydrate Sequence
Caseins / chemistry*
Chromatography, High Pressure Liquid
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Spectrometry, Mass, Fast Atom Bombardment
Reg. No./Substance:

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