Document Detail


Variation of minisatellites in chemically induced mutagenesis and in gene amplification.
MedLine Citation:
PMID:  8400716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mutation assay in cultured mammalian cells was developed based on direct analysis of minisatellite DNA. Chinese hamster cells (V79) were mutagenized with nitrosoguanidine and independent colonies were isolated and expanded. DNA fingerprints were then obtained after digestion with HinfI or HaeIII and hybridization with 33.15 and 33.6 probes (Jeffreys et al., 1985). 12 colonies from untreated cells were also analyzed. Digestion with HaeIII and hybridization with 33.15 probe detected the highest frequency of induced variants. The results suggest that minisatellite sequences are hypermutable sites that can be used to monitor the mutagenic effect of chemical agents. We have also analyzed the DNA fingerprints of 17 independent Chinese hamster (CHO) cell lines carrying amplification of the CAD gene. The DNA fingerprint analysis showed a variation in minisatellite regions in 3 lines while no variation was observed in independent colonies from the CHO parental cell line. The results suggest that these sequences may be hot spots for recombination during gene amplification.
Authors:
P Vagnarelli; E Giulotto; P Fattorini; E Mucciolo; M Bensi; L Tessera; L De Carli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  EXS     Volume:  67     ISSN:  1023-294X     ISO Abbreviation:  EXS     Publication Date:  1993  
Date Detail:
Created Date:  1993-11-05     Completed Date:  1993-11-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9204529     Medline TA:  EXS     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  71-7     Citation Subset:  IM    
Affiliation:
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Line
Cricetinae
DNA Fingerprinting
DNA, Satellite / genetics*
Gene Amplification
Genetic Variation*
Mutagenesis
Nitrosoguanidines
Repetitive Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA, Satellite; 0/Nitrosoguanidines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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