Document Detail

Variation in storage alpha-polyglucans of red algae: amylose and semi-amylopectin types in Porphyridium and glycogen type in Cyanidium.
MedLine Citation:
PMID:  17160635     Owner:  NLM     Status:  MEDLINE    
Red algae are widely known to produce floridean starch but it remains unclear whether the molecular structure of this algal polyglucan is distinct from that of the starch synthesized by vascular plants and green algae. The present study shows that the unicellular species Porphyridium purpureum R-1 (order Porphyridiales, class Bangiophyceae) produces both amylopectin-type and amylose-type alpha-polyglucans. In contrast, Cyanidium caldarium (order Porphyridiales, class Bangiophyceae) synthesizes glycogen-type polyglucan, but not amylose. Detailed analysis of alpha-1,4-chain length distribution of P. purpureum polyglucan suggests that the branched polyglucan has a less ordered structure, referred to as semi-amylopectin, as compared with amylopectin of rice endosperm having a tandem-cluster structure. The P. purpureum linear amylose-type polyglucan, which has a lambda(max) of 630 nm typical of amylose-iodine complex and is resistant to Pseudomonas isoamylase digestion, accounts for less than 10% of the total polyglucans. We produced and isolated a cDNA encoding a granule-bound starch synthase (GBSS)-type protein of P. purpureum, which is probably the approximately 60-kDa protein bound tightly to the starch granules, resembling the amylose-synthesizing GBSS protein of green plants. The present investigation indicates that the class Bangiophyceae includes species producing both semi-amylopectin and amylose, and species producing glycogen alone.
Takahiro Shimonaga; Shoko Fujiwara; Miki Kaneko; Asako Izumo; Satoko Nihei; Perigio B Francisco; Aya Satoh; Naoko Fujita; Yasunori Nakamura; Mikio Tsuzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-12
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  9     ISSN:  1436-2228     ISO Abbreviation:  Mar. Biotechnol.     Publication Date:    2007 Mar-Apr
Date Detail:
Created Date:  2007-04-26     Completed Date:  2007-10-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  192-202     Citation Subset:  IM    
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
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MeSH Terms
Algae, Red / chemistry,  physiology*
Algal Proteins / analysis
Amino Acid Sequence
Amylopectin / chemistry*
Amylose / chemistry*
Glucans / chemistry,  isolation & purification
Glycogen / chemistry*
Molecular Sequence Data
Porphyridium / chemistry,  physiology
Sequence Alignment
Starch Synthase / analysis,  chemistry,  genetics
X-Ray Diffraction
Reg. No./Substance:
0/Algal Proteins; 0/Glucans; 9005-79-2/Glycogen; 9005-82-7/Amylose; 9037-22-3/Amylopectin; EC Synthase

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