Document Detail

Variation in platelet function testing has a major influence on detection of aspirin resistance in healthy subjects.
MedLine Citation:
PMID:  19127088     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: An increased demand for monitoring aspirin treatment by platelet function tests has been observed, but data on the biological variation of these tests are insufficient. The aim of this study was to assess the biological variation of optical platelet aggregometry and closure time in healthy subjects without aspirin and after aspirin ingestion. SUBJECTS AND METHODS: In 20 healthy subjects, blood was sampled 4 times: on 2 consecutive mornings a day after aspirin ingestion (100 mg/daily) and on 2 consecutive days of no treatment. In all samples, arachidonic acid-, ADP- and collagen-induced optical platelet aggregation was measured, and closure times were determined by collagen/epinephrine (CEPI) and collagen/ADP (CADP) cartridges in a platelet function analyzer-100. RESULTS: Aspirin significantly reduced arachidonic acid- and ADP-induced platelet aggregation and significantly prolonged CEPI closure time, but had no significant effect on collagen-induced platelet aggregation and CADP closure time. Aspirin increased both within- and between-subject coefficients of variation. Arachidonic acid-induced platelet aggregation was the most sensitive to aspirin and no aspirin-resistant subjects were detected on either day after aspirin. According to ADP-induced platelet aggregation or CEPI closure time, 25 and 30% of healthy subjects, respectively, changed from aspirin resistant to aspirin responsive or vice versa from one day to another. There was no agreement between platelet function tests in determining aspirin resistance. CONCLUSIONS: A significant variation in optical platelet aggregometry and closure time exists and is presumed to have a major effect on determination of aspirin resistance.
Mojca Bozic-Mijovski; Matej Rakusa; Mojca Stegnar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-05
Journal Detail:
Title:  Pathophysiology of haemostasis and thrombosis     Volume:  36     ISSN:  1424-8840     ISO Abbreviation:  Pathophysiol. Haemost. Thromb.     Publication Date:  2008  
Date Detail:
Created Date:  2009-01-07     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101142710     Medline TA:  Pathophysiol Haemost Thromb     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  84-90     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Department of Vascular Diseases, University Medical Centre, Ljubljana, Slovenia.
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MeSH Terms
Aspirin / administration & dosage,  pharmacokinetics*
Bleeding Time
Diagnostic Errors
Drug Monitoring
Drug Resistance*
Nephelometry and Turbidimetry
Platelet Aggregation / drug effects*
Platelet Function Tests / standards*
Reproducibility of Results
Reg. No./Substance:

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