Document Detail


Variation in the ovarian reserve is linked to alterations in intrafollicular estradiol production and ovarian biomarkers of follicular differentiation and oocyte quality in cattle.
MedLine Citation:
PMID:  19164170     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanisms whereby the high variation in numbers of morphologically healthy oocytes and follicles in ovaries (ovarian reserve) may have an impact onovarian function, oocyte quality, and fertility are poorly understood. The objective was to determine whether previously validated biomarkers for follicular differentiation and function, as well as oocyte quality differed between cattle with low versus a high antral follicle count (AFC). Ovaries were removed (n = 5 per group) near the beginning of the nonovulatory follicular wave, before follicles could be identified via ultrasonography as being dominant, from heifers with high versus a low AFC. The F1, F2, and F3 follicles were dissected and diameters determined. Follicular fluid and thecal, granulosal, and cumulus cells and the oocyte were isolated and subjected to biomarker analyses. Although the size and numerous biomarkers of differentiation, such as mRNAs for the gonadotropin receptors, were similar, intrafollicular concentrations of estradiol and the abundance of mRNAs for CYP19A1 in granulosal cells and ESR1, ESR2, and CTSB in cumulus cells were greater, whereas mRNAs for AMH in granulosal cells and TBC1D1 in thecal cells were lower for animals with low versus a high AFC during follicle waves. Hence, variation in the ovarian reserve may have an impact on follicular function and oocyte quality via alterations in intrafollicular estradiol production and expression of key genes involved in follicle-stimulating hormone action (AMH) and estradiol (CYP19A1) production by granulosal cells, function and survival of thecal cells (TBC1D1), responsiveness of cumulus cells to estradiol (ESR1, ESR2), and cumulus cell determinants of oocyte quality (CTSB).
Authors:
J J Ireland; A E Zielak-Steciwko; F Jimenez-Krassel; J Folger; A Bettegowda; D Scheetz; S Walsh; F Mossa; P G Knight; G W Smith; P Lonergan; A C O Evans
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-01-21
Journal Detail:
Title:  Biology of reproduction     Volume:  80     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-29     Completed Date:  2009-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  954-64     Citation Subset:  IM    
Affiliation:
School of Agriculture Food Science and Veterinary Medicine and Conway Institute, University College Dublin, Dublin, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aromatase / genetics
Base Sequence
Biological Markers / metabolism
Cathepsin B / genetics
Cattle
Cell Count
Cell Differentiation
DNA Primers / genetics
Estradiol / biosynthesis*
Female
Follicular Fluid / metabolism
Growth Substances / genetics
Oocytes / cytology*
Ovarian Follicle / cytology,  metabolism
Ovary / cytology*,  metabolism*
RNA, Messenger / genetics,  metabolism
Receptors, Estrogen / genetics
Chemical
Reg. No./Substance:
0/Biological Markers; 0/DNA Primers; 0/Growth Substances; 0/RNA, Messenger; 0/Receptors, Estrogen; 50-28-2/Estradiol; EC 1.14.14.1/Aromatase; EC 3.4.22.1/Cathepsin B

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