Document Detail

Variation in lateral asymmetry of human chromosome 1.
MedLine Citation:
PMID:  679726     Owner:  NLM     Status:  MEDLINE    
Variations in lateral asymmetry of human chromosome 1 were studied in 17 amniotic cell samples and eight blood samples by the 5-bromodeoxyuridine (BrdU) quenching of 4'-6-diamidino-2-phenylindole (DAPI) fluorescence. The size and the relative proportion of the bright fluorescent spots on each chromatid in the heterochromatic region of chromosome 1 (1qh) are variable from different amniotic (or blood) samples after one cycle of BrdU incorporation. However, the particular pattern for a given chromosome 1 is consistent within the individual sample. Size variations were classified into three groups, and variations in the pattern (proportion) of bright fluorescence on each chromatid in the 1qh region were classified into four groups. A preliminary estimate of the type and frequency of lateral asymmetry variations was obtained. These results suggest a high frequency of variability of heterochromatin in the population. The BrdU-DAPI fluorescence technique was found to be very useful for characterizing variations in the 1qh region; variations in organization of heterochromatin DNA with the 1qh region can be detected, and a simple system of nomenclature is proposed for naming the variations in this region.
M S Lin; O S Alfi
Related Documents :
10854346 - Epidemiology and genetics of essential tremor.
1016226 - Genetic control and distribution of leucine aminopeptidase in the cultivated chenopods ...
21718456 - Could submicroscopical chromosomal imbalances cause cono-truncal malformations in twins?
19184476 - Quantitative variation of line-1 sequences in five species and three subspecies of the ...
18997976 - 4-(pyridin-2-yl)thiazol-2-yl thioglycosides as bidentate ligands for oligosaccharide sy...
10982416 - Tetrad analysis in higher plants. a budding technology.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  21     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1978  
Date Detail:
Created Date:  1978-10-27     Completed Date:  1978-10-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amnion / cytology,  ultrastructure
Bromodeoxyuridine / diagnostic use
Chromosomes, Human, 1-3*
Genetic Variation*
Heterochromatin / ultrastructure
Lymphocytes / ultrastructure
Terminology as Topic
Reg. No./Substance:
0/Heterochromatin; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cultured cell isolates of the smooth muscle layer from the rat duodenum
Next Document:  The chromosomes of cockerels (Gallus domesticus) during meiosis.