Document Detail


Variation in lateral asymmetry of human chromosome 1.
MedLine Citation:
PMID:  679726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Variations in lateral asymmetry of human chromosome 1 were studied in 17 amniotic cell samples and eight blood samples by the 5-bromodeoxyuridine (BrdU) quenching of 4'-6-diamidino-2-phenylindole (DAPI) fluorescence. The size and the relative proportion of the bright fluorescent spots on each chromatid in the heterochromatic region of chromosome 1 (1qh) are variable from different amniotic (or blood) samples after one cycle of BrdU incorporation. However, the particular pattern for a given chromosome 1 is consistent within the individual sample. Size variations were classified into three groups, and variations in the pattern (proportion) of bright fluorescence on each chromatid in the 1qh region were classified into four groups. A preliminary estimate of the type and frequency of lateral asymmetry variations was obtained. These results suggest a high frequency of variability of heterochromatin in the population. The BrdU-DAPI fluorescence technique was found to be very useful for characterizing variations in the 1qh region; variations in organization of heterochromatin DNA with the 1qh region can be detected, and a simple system of nomenclature is proposed for naming the variations in this region.
Authors:
M S Lin; O S Alfi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  21     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1978  
Date Detail:
Created Date:  1978-10-27     Completed Date:  1978-10-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amnion / cytology,  ultrastructure
Bromodeoxyuridine / diagnostic use
Chromosomes, Human, 1-3*
Female
Genetic Variation*
Heterochromatin / ultrastructure
Humans
Lymphocytes / ultrastructure
Terminology as Topic
Chemical
Reg. No./Substance:
0/Heterochromatin; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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