| Variation in hydrogen cyanide production between different strains of Pseudomonas aeruginosa. | |
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MedLine Citation:
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PMID: 21273393 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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There is increasing interest in using the cyanogenic properties of Pseudomonas aeruginosa (PA) to develop a non-microbiological method for its detection. Prior to this, the variation in cyanide production between different PA strains needs to be investigated. Hydrogen cyanide (HCN) released into the gas phase by 96 genotyped PA samples was measured using Selected Ion Flow Tube Mass Spectrometry after 24, 48, 72 and 96 hours of incubation. The HCN produced by a range of non-PA cultures and incubated blank plates was also measured. All PA strains produced more HCN than the controls samples which generated extremely low levels. Analysis across all time points demonstrated that non-mucoid samples produced more HCN than the mucoid samples (p=0.003) but this relationship varied according to strain. There were clear differences in the headspace HCN concentration for different strains. Multivariate analysis of headspace HCN for the commonest strains (Liverpool, Midlands1 and Stoke) revealed a significant effect of strain (p<0.001) and a borderline interaction of strain and phenotype (p=0.051). This confirms that all PA strains produced HCN but to varying degrees and will create interest in the possible future clinical applications of the cyanogenic property of PA. |
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Authors:
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F J Gilchrist; A Alcock; J Belcher; M Brady; A Jones; D Smith; P Spanel; K Webb; W Lenney |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-28 |
Journal Detail:
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Title: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology Volume: - ISSN: 1399-3003 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8803460 Medline TA: Eur Respir J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University Hospital of North Staffordshire Newcastle Road, Stoke-on-Trent, ST4 6QG UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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