Document Detail


Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors.
MedLine Citation:
PMID:  21262117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During recent years, the composition of the gut microbiota (GM) has received increasing attention as a factor in the development of experimental inflammatory disease in animal models. Because increased variation in the GM might lead to increased variation in disease parameters, determining and reducing GM variation between laboratory animals may provide more consistent models. Both genetic and environmental aspects influence the composition of the GM and may vary between laboratory animal breeding centers and within an individual breeding center. This study investigated the variation in cecal microbiota in 8-wk-old NMRI and C57BL/6 mice by using denaturing gradient gel electrophoresis to profile PCR-derived amplicons from bacterial 16S rRNA genes. Comparison of the cecal microbiotas revealed that the similarity index of the inbred C57BL/6Sca strain was 10% higher than that of the outbred Sca:NMRI stock. Comparing C57BL/6 mice from 2 vendors revealed significant differences in the microbial profile, whereas the profiles of C57BL/6Sca mice raised in separate rooms within the same breeding center were not significantly different. Furthermore, housing in individually ventilated cages did not lead to intercage variation. These results show that denaturing gradient gel electrophoresis is a simple tool that can be used to characterize the gut microbiota of mice. Including such characterizations in future quality-control programs may increase the reproducibility of mouse studies.
Authors:
Majbritt Ravn Hufeldt; Dennis S Nielsen; Finn Kvist Vogensen; Tore Midtvedt; Axel Kornerup Hansen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative medicine     Volume:  60     ISSN:  1532-0820     ISO Abbreviation:  Comp. Med.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2011-01-25     Completed Date:  2011-06-23     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  100900466     Medline TA:  Comp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  336-47     Citation Subset:  IM    
Affiliation:
Centre for Applied Laboratory Animal Research, Department of Veterinary Disease Biology, Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cecum / microbiology*
Cluster Analysis
Denaturing Gradient Gel Electrophoresis
Housing, Animal
Mice / genetics,  microbiology*
Mice, Inbred C57BL
Principal Component Analysis
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