Document Detail


Variation of glucoraphanin metabolism in vivo and ex vivo by human gut bacteria.
MedLine Citation:
PMID:  21342607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glucosinolates, phytochemicals found in cruciferous vegetables, are metabolised to bioactive isothiocyanates (ITC) by certain bacteria in the human gut. Substantial individual variation in urinary ITC excretion has been observed in previous cruciferous vegetable-feeding studies. We hypothesised that individual differences in gut microbial community contribute to the observed variation in glucosinolate metabolism, i.e. gut microbiota composition between high- and low-ITC excreters differs. We recruited twenty-three healthy individuals and fed them a standardised meal containing 200 g of cooked broccoli. After the meal, 24 h urinary ITC excretion was measured. Study participants with the highest (n 5) and lowest (n 5) ITC excretion provided faecal samples for ex vivo bacterial cultivation with 50 μm-glucoraphanin, the major glucosinolate found in broccoli. When grown ex vivo, faecal bacteria from the selected high-ITC excreters were able to degrade more glucoraphanin than those from the low-ITC excreters (P = 0·05). However, bacterial fingerprints of faecal and ex vivo culture microbiota revealed no statistically significant differences between the high- and low-ITC excreters in terminal restriction fragment length polymorphism analysis of the bacterial 16S ribosomal RNA gene. In conclusion, glucosinolate degradation by faecal bacteria ex vivo may be associated with in vivo bacterial glucosinolate metabolism capacity, but no direct link to specific bacterial species could be established, possibly due to the complexity and functional redundancy of the gut microbiota.
Authors:
Fei Li; Meredith A J Hullar; Shirley A A Beresford; Johanna W Lampe
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, N.I.H., Extramural     Date:  2011-02-23
Journal Detail:
Title:  The British journal of nutrition     Volume:  106     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-13     Completed Date:  2011-09-19     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  408-16     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Bacteria / genetics,  metabolism*
Brassica / chemistry*
Cooking
Feces / microbiology*
Female
Genes, Bacterial
Glucosinolates / metabolism*
Humans
Imidoesters / metabolism*
Inflorescence
Intestines / metabolism,  microbiology*
Isothiocyanates / urine*
Male
Middle Aged
Polymorphism, Genetic
RNA, Ribosomal, 16S / genetics
Young Adult
Grant Support
ID/Acronym/Agency:
R01 CA070913/CA/NCI NIH HHS; R01 CA070913/CA/NCI NIH HHS; R01 CA070913-05/CA/NCI NIH HHS; R56 CA070913/CA/NCI NIH HHS; R56 CA070913/CA/NCI NIH HHS; R56 CA070913-09/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Glucosinolates; 0/Imidoesters; 0/Isothiocyanates; 0/RNA, Ribosomal, 16S; 0/glucoraphanin; 3129-90-6/isothiocyanic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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