Document Detail


Variation of esterase electromorphs associated with bloodmeal digestion in phlebotomine sand flies (Diptera: Psychodidae).
MedLine Citation:
PMID:  12349853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Esterases are often used to measure genetic variation, yet they may be influenced by external factors. Isoelectrofocusing was used to investigate the effects of bloodmeal on the esterase variation in wild populations of Phlebotomus (Larroussius) ariasi Tonnoir and P. (L.) perniciosus Newstead and in a colonized population of P. (Phlebotomus) duboscqi Neveu-Lemaire. Modified electrophoretic profiles were demonstrated to be associated with blood digestion. The bloodmeal-induced esterase electromorphs were only found in the abdomen homogenates of the sand flies. These changes in esterase phenotype were probably due to a proteolysis phenomenon and disappeared at the end of bloodmeal digestion. The effect was eliminated by adding trypsin inhibitors.
Authors:
S Perrotey; B Klein; G Madulo-Leblond; M Gállego; B Pesson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical entomology     Volume:  39     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-27     Completed Date:  2002-12-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  720-2     Citation Subset:  IM    
Affiliation:
Faculté de Pharmacie, Université Strasbourg I, Illkirch, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Digestion
Esterases / analysis*
Female
Genetic Variation
Humans
Isoelectric Focusing / methods
Phenotype
Psychodidae / enzymology*,  metabolism
Chemical
Reg. No./Substance:
EC 3.1.-/Esterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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