Document Detail

Variation of the HES concentration for the cryopreservation of keratinocytes in suspensions and in monolayers.
MedLine Citation:
PMID:  11034787     Owner:  NLM     Status:  MEDLINE    
It has recently been shown that keratinocytes, both in suspension and in monolayers, can be successfully cryopreserved with hydroxyethyl starch (HES) (6, 9). HES is a nontoxic biodegradable macromolecule which is clinically approved as a plasma expander and which has already been used for the cryopreservation of red blood cells (10, 11). In this study we varied the HES concentration between 0 and 10 wt% in 2% steps for suspended cells and between 0, 4, 6, 8, and 10 wt% for monolayer cells in order to determine the effect on the survival rate and metabolic activity after cryopreservation. The experiments with the suspended cells were performed both with and without NCS. Cryopreserved keratinocytes can be transplanted onto patients for the treatment of deep dermal burns and leg ulcers. In this study, we achieved a survival rate of 80% for the suspended cells (10 wt% HES, 3 degrees C/min) and a survival rate of even 88% when the cells were cryopreserved as a monolayer using the same parameters. The addition of NCS did not improve the results for the suspended cells significantly.
J Pasch; A Schiefer; I Heschel; N Dimoudis; G Rau
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  41     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-12-26     Completed Date:  2000-12-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  89-96     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Helmholtz-Institute for Biomedical Engineering at the Aachen University of Technology (RWTH), Pauwelsstr. 20, D-52074 Aachen, Germany.
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MeSH Terms
Cell Culture Techniques
Cell Survival
Cells, Cultured
Cryoprotective Agents / pharmacology*
Culture Media, Serum-Free
Dose-Response Relationship, Drug
Fetal Blood / physiology
Hetastarch / pharmacology*
Keratinocytes / cytology,  drug effects*
Osmolar Concentration
Reg. No./Substance:
0/Cryoprotective Agents; 0/Culture Media, Serum-Free; 0/Suspensions; 9005-27-0/Hetastarch

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