| Variation of the 3' telomeric overhang lengths in human cells. | |
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MedLine Citation:
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PMID: 18316158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The telomeric G-rich 3' overhang is important for the maintenance of chromosomal integrity by stabilizing T-loop structure in which the 3' overhang invades the double-stranded telomeric DNA. However, the 3' overhang length has not been examined in different human cell lines, and its regulatory mechanism has not been revealed. In this study, we examined overhang length in 56 human cancerous cell lines and five normal cell lines, originated from various tissues. In cancer cells, relative overhang length existed in a wide range from 23% to 308% and showed no significant association with tissue types although short overhang was noted in brain, cervix, and colorectal cells. Normal cells exhibited overhangs in the range from 92% to 202%, which were relatively longer than those seen in cancer cells (p = 0.002). The overhang length was positively correlated with telomere length (p < 0.001), and showed no correlation with mRNA levels of hTERT, a catalytic protein of telomerase, POT1, an overhang binding protein and TPP1, a POT1 interacting protein. This study demonstrates a broad distribution of overhang length in human cells, suggesting a dynamic regulation of 3' overhang length. The overhang length seems to be closely associated with telomere length and might be regulated by multiple mechanisms. |
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Authors:
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Myung Eun Lee; Sun Young Rha; Hei-Cheul Jeung; Tae Soo Kim; Hyun Cheol Chung; Bong-Kyeong Oh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-07 |
Journal Detail:
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Title: Cancer letters Volume: 264 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-04-29 Completed Date: 2008-07-24 Revised Date: 2009-10-06 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 107-18 Citation Subset: IM |
Affiliation:
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Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3' Flanking Region
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genetics* Cell Line, Tumor Cells, Cultured Colon / cytology, metabolism Endothelial Cells / cytology, metabolism Genetic Variation* Humans In Situ Hybridization Lung / cytology, metabolism Neoplasms / genetics*, metabolism, pathology RNA, Messenger / analysis, biosynthesis Telomerase / genetics Telomere / chemistry, genetics* Telomere-Binding Proteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/POT1 protein, human; 0/PTOP protein, human; 0/RNA, Messenger; 0/Telomere-Binding Proteins; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
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