Document Detail


Variant forms of plasma apolipoprotein B. Hepatic and intestinal biosynthesis and heterogeneous metabolism in the rat.
MedLine Citation:
PMID:  7217048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies of circulating lipoproteins in man and the rat have shown that apolipoprotein B in plasma exists in two immunologically distinct forms with apparent Mr, in the rat, of approximately 335,000 (apoB335K) and 240,000 (apoB240K). With isolated, perfused organs and single and double isotope labeling methods in vivo (1979) J. Biol. Chem. 254, 7316), we have now found that the rat liver produces both apoB variants in abundance while the small intestine produces apoB240K but only trace amounts of apoB335K. The hepatic apoB335K/apoB240Kleucine incorporation ratio ranged from 0.5-1.7 under different conditions, suggesting physiological regulation of the relative amounts produced. Disappearance of both newly synthesized hepatic apoB variants from plasma followed first order kinetics. The circulating half-life of hepatic apoB335K was 2.4 times that of hepatic apoB240K, indicating independent catabolism of the two variants and consistent with the hepatic production of multiple apoB-containing lipoproteins with different metabolic properties. The half-life of apoB240K from the intestine was even shorter than that of apoB240K from the liver.
Authors:
A L Wu; H G Windmueller
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  256     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-06-23     Completed Date:  1981-06-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3615-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins / biosynthesis*,  genetics
Apolipoproteins B
Genetic Variation*
Intestine, Small / metabolism*
Kinetics
Liver / metabolism*
Male
Molecular Weight
Organ Specificity
Rats
Chemical
Reg. No./Substance:
0/Apolipoproteins; 0/Apolipoproteins B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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