Document Detail

Variables in xanthine oxidase-initiated luminol chemiluminescence: implications for chemiluminescence measurements in biological systems.
MedLine Citation:
PMID:  3799963     Owner:  NLM     Status:  MEDLINE    
We tested the effects of generally used chemiluminescence inhibitors on an example of luminol chemiluminescence elicited by xanthine oxidase/hypoxanthine system, and attempted to assess their capabilities in discovering the reaction pathways leading to chemiluminescence. Luminol itself is a xanthine oxidase inhibitor and its concentration affects the reaction mechanism. Maximal chemiluminescence response was observed at luminol concentration inhibiting urate production. Chemiluminescence was totally inhibited by superoxide dismutase, the inhibition by catalase depended on luminol concentration. Ferricytochrome c, a detector of superoxide, either stimulated or inhibited chemiluminescence in a concentration-dependent manner. Chemiluminescence was highly stimulated by peroxidases. A pronounced inhibition of chemiluminescence was caused by chelators; 1 mM desferal and 0.01 mM diethyldithiocarbamate. It is suggested that measurement of luminol chemiluminescence is not a suitable method for discrimination among individual reactive oxygen species and their quantitative determination in biological systems.
J Wilhelm; V Vilím
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  158     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1987-01-30     Completed Date:  1987-01-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  201-10     Citation Subset:  IM    
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MeSH Terms
Hydrogen Peroxide
Luminescent Measurements*
Superoxide Dismutase
Xanthine Oxidase* / antagonists & inhibitors
Reg. No./Substance:
0/Hypoxanthines; 0/Pyridazines; 521-31-3/Luminol; 68-94-0/Hypoxanthine; 7722-84-1/Hydrogen Peroxide; EC; EC Dismutase; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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