Document Detail


Variable restriction of albumin diffusion across inflamed cerebral microvessels of the anaesthetized rat.
MedLine Citation:
PMID:  8189387     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The possibility of restricted diffusion of macromolecules in single cerebral venular capillaries that have become leaky due to inflammation was investigated by comparing the permeabilities to Lucifer Yellow (457 Da; PLY) and rhodamine-labelled albumin (69 kDa; PRh-A). 2. The dyes were trapped between two micro-occlusion probes and the permeabilities were measured from the rates of decrease in dye fluorescence at low intraluminal hydrostatic pressure. 3. Removal of one probe had little effect on PLY but did reduce PRh-A, consistent with the influence of convection on diffusion through 22 nm wide transendothelial slits 1 micron deep. 4. Direct comparisons were made over time between PLY and PRh-A in six vessels while hydrostatic pressure effects were controlled. In all vessels PRh-A:PLY varied from being similar to the ratio of the free diffusion coefficients to virtually zero even though PLY remained high. The question of the source of this variable restriction to albumin is discussed in terms of the secretion and sloughing of glycosaminoglycans and the possible role of transient formation of transendothelial gaps.
Authors:
A S Easton; P A Fraser
Related Documents :
511687 - Increased lung lymph transport without heart failure after coronary ligation in sheep.
7278197 - Vesicle transport in arterial endothelium and the influence of mechanical factors on ma...
8416417 - Effects of continuous infusion of nitroglycerin on pulmonary hemodynamics, lung lymph b...
7505487 - Hyskon-induced pulmonary edema.
12675277 - Factors relating to inadequate control of blood pressure in hypertensive outpatients.
9404797 - Secondary acute angle closure glaucoma: a complication of aids.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  475     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-06-23     Completed Date:  1994-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  147-57     Citation Subset:  IM; S    
Affiliation:
Vascular Biology Research Centre Physiology Group, King's College London.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Albumins / metabolism*
Anesthesia*
Animals
Blood-Brain Barrier / physiology*
Capillary Permeability / physiology*
Diffusion
Fluorescent Dyes
Histocytochemistry
Isoquinolines
Meninges / metabolism
Pressure
Rats
Rats, Wistar
Rhodamines
Solvents
Vasculitis / metabolism*
Chemical
Reg. No./Substance:
0/Albumins; 0/Fluorescent Dyes; 0/Isoquinolines; 0/Rhodamines; 0/Solvents; 77944-88-8/lucifer yellow
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  ACTH response induced in capsaicin-desensitized rats by intravenous injection of interleukin-1 or pr...
Next Document:  Intrarenal haemodynamic and glomerular responses to inhibition of nitric oxide formation in rabbits.