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Variable pathways for developmental changes of mitosis and cytokinesis in Physarum polycephalum.
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MedLine Citation:
PMID:  2016339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of a uninucleate ameba into a multinucleate, syncytial plasmodium in myxomycetes involves a change from the open, astral mitosis of the ameba to the intranuclear, anastral mitosis of the plasmodium, and the omission of cytokinesis from the cell cycle. We describe immunofluorescence microscopic studies of the amebal-plasmodial transition (APT) in Physarum polycephalum. We demonstrate that the reorganization of mitotic spindles commences in uninucleate cells after commitment to plasmodium formation, is completed by the binucleate stage, and occurs via different routes in individual developing cells. Most uninucleate developing cells formed mitotic spindles characteristic either of amebae or of plasmodia. However, chimeric mitotic figures exhibiting features of both amebal and plasmodial mitoses, and a novel star microtubular array were also observed. The loss of the ameba-specific alpha 3-tubulin and the accumulation of the plasmodium-specific beta 2-tubulin isotypes during development were not sufficient to explain the changes in the organization of mitotic spindles. The majority of uninucleate developing cells undergoing astral mitoses (amebal and chimeric) exhibited cytokinetic furrows, whereas cells with the anastral plasmodial mitosis exhibited no furrows. Thus, the transition from astral to anastral mitosis during the APT could be sufficient for the omission of cytokinesis from the cell cycle. However, astral mitosis may not ensure cytokinesis: some cells undergoing amebal or chimeric mitosis contained unilateral cytokinetic furrows or no furrow at all. These cells would, most probably, fail to divide. We suggest that a uninucleate committed cell undergoing amebal or chimeric mitosis can either divide or else form a binucleate cell. In contrast, a uninucleate cell with a mitotic spindle of the plasmodial type gives rise only to a binucleate cells. Further, the decision to enter mitosis after commitment to the APT is independent of the developmental changes in the organization of the mitotic spindle and cytokinesis.
Authors:
L Solnica-Krezel; T G Burland; W F Dove
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  113     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-05-21     Completed Date:  1991-05-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  591-604     Citation Subset:  IM    
Affiliation:
McArdle Laboratory for Cancer Research, Madison, Wisconsin 53706.
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MeSH Terms
Descriptor/Qualifier:
Cell Division*
Fluorescent Antibody Technique
Microtubules / chemistry,  ultrastructure*
Mitosis*
Mitotic Spindle Apparatus / chemistry,  ultrastructure*
Physarum / analysis,  cytology*,  ultrastructure
Tubulin / analysis
Grant Support
ID/Acronym/Agency:
CA-23076/CA/NCI NIH HHS; GA-07175//PHS HHS
Chemical
Reg. No./Substance:
0/Tubulin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1991
Volume: 113 Issue: 3
First Page: 591 Last Page: 604
ID: 2288956
Publisher Id: 91201409
PubMed Id: 2016339

Variable pathways for developmental changes of mitosis and cytokinesis in Physarum polycephalum


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